<?xml version="1.0" encoding="UTF-8" standalone="yes"?>

<!-- Copyright (c) 2004-2026 Broad Institute, Inc., Massachusetts Institute of Technology, and Regents of the University of California.  All rights reserved.
     See license terms at www.gsea-msigdb.org/gsea/license_terms_list. Please note that certain gene sets have special access terms.
-->
<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="LIU_TARGETS_OF_VMYB_VS_CMYB_UP" SYSTEMATIC_NAME="M3389" HISTORICAL_NAMES="" PMID="16205643" AUTHORS="Liu F,Lei W,O'Rourke JP,Ness SA" GEOID="GSE2815,GSE2816" EXACT_SOURCE="Table 4S: Heat Map Order 1-27" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_HG_U133" CONTRIBUTOR="Arthur Liberzon" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes regulated in the opposite directions by v-MYB (UP) and c-MYB  (DN) variants of CMYB [GeneID=4602] overexpressed in primary monocyte cultures off adenoviral vectors." DESCRIPTION_FULL="The v-Myb oncoprotein encoded by Avian Myeloblastosis Virus is highly oncogenic, induces leukemias in chickens and mice and transforms immature hematopoietic cells in vitro. The v-Myb protein is a mutated and truncated version of c-Myb, a DNA-binding transcription factor expressed in many cell types that is essential for normal hematopoiesis. Previous studies suggested that two types of differences, DNA binding domain mutations and the deletion of a C-terminal negative regulatory domain were important for increasing the transforming activity of v-Myb. Here, we combined structure-function studies of the v-Myb and c-Myb proteins with unbiased microarray-based transcription assays to compare the transcriptional specificities of the two proteins. In human cells, the v-Myb and c-Myb proteins displayed strikingly different activities and regulated overlapping, but largely distinct sets of target genes. Each type of mutation that distinguished v-Myb from c-Myb, including the N- and C-terminal deletions, DNA binding domain changes and mutations in the transcriptional activation domain, affected different sets of target genes and contributed to the different activities of c-Myb and v-Myb. The results suggest that v-Myb is not just a de-repressed version of c-Myb. Instead, it is a distinct transcriptional regulator with a unique set of activities." TAGS="" MEMBERS="1552587_at,1555836_at,1556722_a_at,1559169_at,1562849_at,1567078_x_at,201847_at,204834_at,219645_at,220082_at,220596_at,222868_s_at,228613_at,228949_at,229229_at,229675_at,230739_at,230746_s_at,232831_at,234074_at,234870_at,235209_at,235831_at,236486_at,238124_at,239719_at,244605_at" MEMBERS_SYMBOLIZED="AGXT2,C20orf203,CASQ1,CD109,CNBD1,FAM210A,FGL2,IL18BP,LIPA,MYOM3,NAXE,PPP1R14D,RAB11FIP3,SBSPON,WLS,ZNF385D" MEMBERS_EZID="10068,10875,125228,127294,128240,135228,157869,168975,284805,3988,54866,64902,79750,79971,844,9727" MEMBERS_MAPPING="1552587_at,CNBD1,168975|1555836_at,null,null|1556722_a_at,C20orf203,284805|1559169_at,null,null|1562849_at,null,null|1567078_x_at,null,null|201847_at,LIPA,3988|204834_at,FGL2,10875|219645_at,CASQ1,844|220082_at,PPP1R14D,54866|220596_at,NAXE,128240|222868_s_at,IL18BP,10068|228613_at,RAB11FIP3,9727|228949_at,WLS,79971|229229_at,AGXT2,64902|229675_at,null,null|230739_at,FAM210A,125228|230746_s_at,null,null|232831_at,null,null|234074_at,null,null|234870_at,null,null|235209_at,SBSPON,157869|235831_at,ZNF385D,79750|236486_at,null,null|238124_at,MYOM3,127294|239719_at,CD109,135228|244605_at,null,null" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Homo sapiens" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
