STANDARD_NAME	LOPEZ_MBD_TARGETS_IMPRINTED_AND_X_LINKED
SYSTEMATIC_NAME	M4324
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/LOPEZ_MBD_TARGETS_IMPRINTED_AND_X_LINKED
NAMESPACE	HUMAN_GENE_SYMBOL
DESCRIPTION_BRIEF	X chromosome and imprinted genes up-regulated in HeLa cells (cervical cancer) after knockdown of the MBD (methyl-CpG binding domain) proteins by RNAi.
DESCRIPTION_FULL	Methyl-cytosine-phosphate-guanine (CpG)-binding domain (MBD) proteins are bound to hypermethylated promoter CpG islands of tumor suppressor genes in human cancer cells, although a direct causal relationship at the genome-wide level between MBD presence and gene silencing remains to be demonstrated. To this end, we have inhibited the expression of MBD proteins in HeLa cells by short hairpin RNAs; and studied the functional consequences of MBD depletion using microarray-based expression analysis in conjunction with extensive bisulfite genomic sequencing and chromatin immunoprecipitation. The removal of MBDs results in a release of gene silencing associated with a loss of MBD occupancy in 5'-CpG islands without any change in the DNA methylation pattern. Our results unveil new targets for epigenetic inactivation mediated by MBDs in transformed cells, such as the cell adhesion protein gamma-parvin and the fibroblast growth factor 19, where we also demonstrate their bona fide tumor suppressor features. Our data support a fundamental role for MBD proteins in the direct maintenance of transcriptional repression of tumor suppressors and identify new candidate genes for epigenetic disruption in cancer cells.
PMID	18223687
GEOID	
AUTHORS	Lopez-Serra L,Ballestar E,Ropero S,Setien F,Billard LM,Fraga MF,Lopez-Nieva P,Alaminos M,Guerrero D,Dante R,Esteller M
CONTRIBUTOR	Jessica Robertson
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 2S
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	ABCD1,EIF1AX,FAM3A,GPC3,HDGF,IDH3G,IGF2,IL3RA,IRAK1,MAGEA6,MEG3,MTM1,NXT2,PDHA1,SDHD,UBE1,UBE2A
GENE_SYMBOLS	ABCD1,EIF1AX,FAM3A,GPC3,HDGF,IDH3G,IGF2,IL3RA,IRAK1,MAGEA6,MEG3,MTM1,NXT2,PDHA1,SDHD,UBA1,UBE2A
FOUNDER_NAMES