STANDARD_NAME MIYAGAWA_TARGETS_OF_EWSR1_ETS_FUSIONS_UP SYSTEMATIC_NAME M2255 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/MIYAGAWA_TARGETS_OF_EWSR1_ETS_FUSIONS_UP NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes commonly up-regulated in UET-13 cells (mesenchymal progenitor) by expression of EWSR1 [GeneID=2130] fusions with ETS transcription factors FLI1 and ERG [GeneID=2313 ,2078]. DESCRIPTION_FULL Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in the onset and progression of EFT. However, the mechanisms responsible for the EWS/ETS-mediated onset remain unclear. Here we report the establishment of a tetracycline-controlled EWS/ETS-inducible system in human bone marrow-derived mesenchymal progenitor cells (MPCs). Ectopic expression of both EWS/FLI1 and EWS/ERG proteins resulted in a dramatic change of morphology, i.e., from a mesenchymal spindle shape to a small round-to-polygonal cell, one of the characteristics of EFT. EWS/ETS also induced immunophenotypic changes in MPCs, including the disappearance of the mesenchyme-positive markers CD10 and CD13 and the up-regulation of the EFT-positive markers CD54, CD99, CD117, and CD271. Furthermore, a prominent shift from the gene expression profile of MPCs to that of EFT was observed in the presence of EWS/ETS. Together with the observation that EWS/ETS enhances the ability of cells to invade Matrigel, these results suggest that EWS/ETS proteins contribute to alterations of cellular features and confer an EFT-like phenotype to human MPCs. PMID 18212050 GEOID GSE8596,GSE8665 AUTHORS Miyagawa Y,Okita H,Nakaijima H,Horiuchi Y,Sato B,Taguchi T,Toyoda M,Katagiri YU,Fujimoto J,Hata J,Umezawa A,Kiyokawa N CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 1S FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1438_at,1552507_at,1552508_at,1552610_a_at,1554062_at,1554384_at,1554830_a_at,1555705_a_at,1555779_a_at,1557116_at,1558279_a_at,1568592_at,200672_x_at,200677_at,200762_at,200905_x_at,201028_s_at,201042_at,201288_at,201416_at,201418_s_at,201562_s_at,201563_at,201565_s_at,201566_x_at,201578_at,201596_x_at,201601_x_at,201648_at,201649_at,201650_at,201911_s_at,202008_s_at,202241_at,202419_at,202638_s_at,202711_at,202718_at,202761_s_at,202762_at,202763_at,202803_s_at,202838_at,202897_at,203088_at,203126_at,203139_at,203214_x_at,203217_s_at,203320_at,203358_s_at,203397_s_at,203499_at,203615_x_at,203620_s_at,203705_s_at,203706_s_at,203716_s_at,203865_s_at,203964_at,204088_at,204137_at,204149_s_at,204201_s_at,204224_s_at,204229_at,204230_s_at,204319_s_at,204394_at,204395_s_at,204396_s_at,204497_at,204548_at,204550_x_at,204600_at,204604_at,204633_s_at,204683_at,204743_at,204761_at,204780_s_at,204781_s_at,204818_at,204899_s_at,204917_s_at,205049_s_at,205051_s_at,205173_x_at,205227_at,205249_at,205306_x_at,205375_at,205450_at,205524_s_at,205542_at,205567_at,205575_at,205637_s_at,205771_s_at,205779_at,205786_s_at,205817_at,205824_at,205828_at,205858_at,205897_at,206060_s_at,206078_at,206231_at,206561_s_at,206655_s_at,206665_s_at,206915_at,208072_s_at,208096_s_at,208763_s_at,208891_at,208892_s_at,208893_s_at,208934_s_at,208936_x_at,208986_at,209099_x_at,209283_at,209373_at,209496_at,209498_at,209539_at,209558_s_at,209610_s_at,209611_s_at,209616_s_at,209652_s_at,209679_s_at,209710_at,209791_at,209921_at,209962_at,209963_s_at,210058_at,210059_s_at,210186_s_at,210257_x_at,210358_x_at,210651_s_at,210786_s_at,210912_x_at,210960_at,210961_s_at,210973_s_at,211003_x_at,211138_s_at,211214_s_at,211478_s_at,211502_s_at,211709_s_at,211825_s_at,212045_at,212098_at,212192_at,212288_at,212293_at,212312_at,212486_s_at,212612_at,212670_at,212810_s_at,212811_x_at,212956_at,212960_at,213040_s_at,213201_s_at,213316_at,213524_s_at,213552_at,213620_s_at,214119_s_at,215034_s_at,215054_at,215719_x_at,215856_at,216033_s_at,216252_x_at,216268_s_at,216548_x_at,217678_at,217783_s_at,217979_at,217992_s_at,218177_at,218424_s_at,218559_s_at,218665_at,218826_at,218976_at,219032_x_at,219054_at,219102_at,219218_at,219371_s_at,219471_at,219520_s_at,219576_at,220102_at,220486_x_at,220948_s_at,221011_s_at,221039_s_at,221530_s_at,221552_at,221679_s_at,221710_x_at,221718_s_at,222106_at,222379_at,222408_s_at,222482_at,222483_at,222549_at,222670_s_at,222760_at,223093_at,223177_at,223178_s_at,223202_s_at,223437_at,223557_s_at,223689_at,223721_s_at,223734_at,224325_at,224748_at,224790_at,224796_at,224884_at,224909_s_at,224925_at,224959_at,224963_at,225051_at,225079_at,225095_at,225140_at,225177_at,225186_at,225189_s_at,225355_at,225447_at,225510_at,225531_at,225564_at,225601_at,225871_at,226055_at,226208_at,226365_at,226408_at,226552_at,226683_at,226702_at,226741_at,226908_at,226932_at,227058_at,227115_at,227188_at,227224_at,227361_at,227377_at,227385_at,227401_at,227405_s_at,227410_at,227490_at,227533_at,227647_at,227663_at,227750_at,227850_x_at,227862_at,228054_at,228159_at,228212_at,228333_at,228339_at,228347_at,228523_at,228737_at,228977_at,229041_s_at,229256_at,229400_at,229622_at,229810_at,229850_at,230108_at,230135_at,230161_at,230356_at,230653_at,230741_at,230800_at,231314_at,231382_at,231714_s_at,231807_at,231841_s_at,231842_at,231899_at,232172_at,233565_s_at,234971_x_at,235012_at,235056_at,235068_at,235123_at,235252_at,235692_at,235874_at,235890_at,235944_at,236029_at,236088_at,236179_at,236218_at,236442_at,236982_at,237270_at,237466_s_at,238041_at,238133_at,238323_at,238417_at,238532_at,238808_at,239135_at,240950_s_at,242051_at,242458_at,242871_at,35666_at,36564_at,37986_at,38340_at,39966_at,45288_at,58308_at,59437_at,61734_at GENE_SYMBOLS EPHB3,KCNE4,KCNE4,JAK1,XG,PADI2,STEAP3,CMTM3,CD79A,APOL6,KDSR,TRIM69,SPTBN1,PTTG1IP,DPYSL2,HLA-E,CD99,TGM2,ARHGDIB,SOX4,SOX4,SORD,SORD,ID2,,PODXL,KRT18,IFITM1,JAK1,UBE2L6,KRT19,FARP1,NID1,TRIB1,KDSR,ICAM1,EFNB1,IGFBP2,SYNE2,ROCK2,CASP3,ITGB2,FUCA1,SIRPA,FBLN5,IMPA2,DAPK1,CDK1,ST3GAL5,SH2B3,EZH2,GALNT3,EPHA2,SULT1A2,FCHSD2,FZD7,FZD7,DPP4,ADARB1,NMI,P2RX4,GPR137B,GSTM4,PTPN13,GCH1,SLC17A7,SLC17A7,RGS10,SLC43A1,GRK5,GRK5,ADCY9,STAR,GSTM1,EPHB3,CDK14,RPS6KA5,ICAM2,TAGLN3,USP6NL,FAS,FAS,HSD17B2,SAP30,MLLT3,CD79A,KIT,CD58,IL1RAP,,KMO,MDFI,PHKA1,HAPLN1,STEAP1,CHST1,C1QL1,SH3GL3,AKAP7,RAMP2,ITGAM,SIX1,HSPB2,MMP3,NGFR,NFATC4,PTPN22,KALRN,KCNN1,AKR1B10,GP1BB,BCL2L1,NKX2-2,DGKD,COL21A1,TSC22D3,DUSP6,DUSP6,DUSP6,LGALS8,LGALS8,TCF12,JAG1,CRYAB,MALL,RARRES2,CEACAM1,ARHGEF6,HIP1R,SLC1A4,SLC1A4,CES1,PGF,SMAGP,GATA2,PADI2,SLC7A11,EPOR,EPOR,MAPK13,MAPK13,FKBP1A,CUL4B,GATA2,EPHB2,FLI1,GSTM4,ADRA1D,ADRA1D,FGFR1,TGM2,KMO,DAPK1,DPP4,CDK14,CLEC11A,FLI1,GLG1,MGAT5,KCTD12,FNBP1,HIPK1,BCL2L1,FYN,RCOR1,ELN,SLC1A4,SLC1A4,TBC1D9,TBC1D9,NPTXR,TNNT1,JCAD,G0S2,GLCE,ICAM2,FKBP1A,TM4SF1,EPOR,FAS,SIGLEC15,FYN,FAS,JAG1,HMGB3P1,SLC7A11,YPEL5,TSPAN13,EFHD2,CHMP1B,STEAP3,MAFB,FZD4,SLC35F2,DNAJC12,OPN3,NPR3,RCN3,BAHCC1,KLF2,RUBCNL,WWC3,MAP7D3,FOXL2,TMEM164,ATP1A1,LBH,ASAP1,BHLHE41,ABHD6,ABHD6,EVA1B,AKAP13,PRND,KCNE4,YPEL5,SSBP3,EFHD2,CLDN1,MAFB,ZNF703,ANKH,NT5DC1,NT5DC1,TMEM164,PPARA,TMEFF2,IGF2BP1,DNAJC12,MGARP,FZD8,DCAF7,ASAP1,ASAP1,AKAP13,PREX1,PREX1,SLC26A2,SLC26A2,EPB41,EMP2,SPTLC2,KLF3,RAB11FIP1,RAPH1,RAPH1,NEURL1B,GPD2,OAF,CABLES1,SPATA13,HMGB3,STEAP2,ARRDC2,ZSWIM6,,TEAD2,IER5L,SNX18,CMPK2,SLC12A6,LRIG3,SSPN,MEDAG,LINC02762,EVA1C,RALGPS2,HS3ST3B1,IGF2BP1,PLPP6,IL17D,FZD8,FAM43A,WDFY2,RALGPS2,KCNE3,,KALRN,CDC42EP5,TRNP1,TMEM44,WDFY2,ISM2,ZEB2,ECSCR,SIX1,NANOS1,TOX2,,ITGB2-AS1,PGM2L1,HOXD10,ERFE,RSPH3,KDSR,ERCC6,HHIP,,NKX1-2,,P2RX7,ADCY4,MRPS22,FGF18,AP4B1,KIAA1217,JCAD,JCAD,ZC3H12C,CD99P1,SDCBP2,PLCD3,LRCH1,ETV6,ZDHHC21,,KSR1,SH3KBP1,PRSS35,TBL1XR1,HMCN1,FAT3,NTNG1,CDH11,PHOSPHO1,DPF3,,ENSG00000272970,HHIP,TCF12,NTNG1,TEAD2,PGM2L1,DPF3,HOXA13,CPPED1,KASH5,,RALGPS2,PAQR5,SEMA3F,RNF19B,EPOR,HIP1R,CSPG5,ABHD6,TRIM62,PIERCE1,RCN3 FOUNDER_NAMES