STANDARD_NAME	NABA_MATRISOME_POORLY_METASTATIC_BREAST_CANCER_TUMOR_CELL_DERIVED
SYSTEMATIC_NAME	M47985
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/NABA_MATRISOME_POORLY_METASTATIC_BREAST_CANCER_TUMOR_CELL_DERIVED
NAMESPACE	HUMAN_GENE_SYMBOL
DESCRIPTION_BRIEF	Tumor-cell-derived matrisome proteins exclusively detected in poorly metastatic breast cancer human-to-mouse xenografts (MDA-MB-231) in comparison to highly metastatic breast cancer human-to-mouse xenografts (MDA-MB-231_LM2)
DESCRIPTION_FULL	To define the ECM composition of tissues and tumors, we have empolyed a proteomics-based method to enrich and identify ECM proteins and coupled it with a bioinformatic annotation of the  matrisome  defined as the ensemble of ECM and ECM-associated proteins. To identify ECM proteins important for breast cancer progression and metastasis formation, we used a xenograft model where human breast cancer cells of differing metastatic potenital were orthotopically injected into the mouse mammary fat pad. The poorly metastatic MDA-MB-231 cell line was established from cells isolated from a sample from a triple-negative breast cancer patient. The highly metastatic MDA-MB-231-LM2 line (denoted LM2), was previously selected and characterized for increased metastatic potential to the lungs. 6.5 weeks post-injection, the primary tumors were harvested, ECM proteins were enriched from tumors, and the composition of the ECM-enriched fractions obtained was characterized by mass spectrometry. We define the matrisome of a tumor as the ensemble of proteins detected in two independent biological replicates and by at least two peptides in one of the two replicates. Using this proteomics approach we show that both the tumor cells and the stromal cells contribute in characteristic ways to the production of the tumor ECM. Moreover, we show that both tumor- and stroma-derived proteins differ between tumors of different metastatic potential. Comparison of the matrisomes of MDA-MB-231 tumors and LM2 tumors identifies ECM proteins characteristic of poorly and highly metastatic tumors. This gene set lists the matrisome proteins secreted by the tumor cells in MDA-MB-231 tumors and not from LM2 tumors.
PMID	24618895
GEOID	MSV000078535
AUTHORS	Naba A,Clauser KR,Lamar JM,Carr SA,Hynes RO
CONTRIBUTOR	Alexandra Naba
CONTRIBUTOR_ORG	University of Illinois Chicago
EXACT_SOURCE	elife-01308-fig3-data1-v1.xlsx, Figure 3C. The tumor extracellular matrix is secreted by both tumor cells and stromal cells and differs with the tumor s metastatic potential.
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	https://doi.org/10.7554/eLife.01308
SOURCE_MEMBERS	COL17A1,COL19A1,EMID2,FBLN5,FLG2,MMP1,MMP19,NGLY1,OGN,PLAT,S100A16,S100A8
GENE_SYMBOLS	COL17A1,COL19A1,COL26A1,FBLN5,FLG2,MMP1,MMP19,NGLY1,OGN,PLAT,S100A16,S100A8
FOUNDER_NAMES