STANDARD_NAME NAKAMURA_ADIPOGENESIS_EARLY_UP SYSTEMATIC_NAME M2024 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/NAKAMURA_ADIPOGENESIS_EARLY_UP NAMESPACE HUMAN_SEQ_ACCESSION DESCRIPTION_BRIEF Genes up-regulated in mesenchymal stem cells during early phase of adipogenesis, defined as days 1 to 5 of culturing with adipogenic hormones. DESCRIPTION_FULL Human bone marrow mesenchymal stem cells (hMSCs) give rise to adipocytes in response to adipogenic hormones. An in-house cDNA microarray representing 3400 genes was employed to characterize the modulation of genes involved in this process. A total of 197 genes showed temporal gene expression changes during adipogenesis, including genes encoding transcriptional regulators and signaling molecules. Semi-quantitative RT-PCR analyses confirmed differential expression at the transcriptional level of several genes identified by cDNA microarray screening. Cluster analysis of the genes regulated during the late phase (from day 7 to day 14) of hMSC adipogenesis indicated that these changes are well correlated with data previously reported for murine preadipocytes. However, during the early phase (day 1-day 5), the modulations of genes differed from those reported for the preadipocytes. These data provide novel information on the molecular mechanisms required for lineage commitment and maturation accompanying adipogenesis of hMSC. PMID 12646203 GEOID AUTHORS Nakamura T,Shiojima S,Hirai Y,Iwama T,Tsuruzoe N,Hirasawa A,Katsuma S,Tsujimoto G CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Fig.2: % of change >80% at days 1,3 or 5 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS AA043494,AA115473,AA132964,AA134749,AA134862,AA187207,AA194765,AA233892,AA253464,AA292054,AA425001,AA442092,AA443982,AA448641,AA449745,AA453978,AA454143,AA457501,AA463460,AA464525,AA464600,AA478436,AA488084,AA504253,AA504354,AA521292,AA621183,AA629542,AA633811,AA679177,AA682819,AA682838,AA700054,AA700772,AA701465,AA707922,AA708440,AA709414,AA776730,AA865960,AA872383,AA872420,AA873182,AA878635,AA912448,AI015412,AI288845,AI289110,H48420,H53340,H57309,H59000,H59620,H72722,H77766,H99829,IMAGE:4971659,N22980,N26658,N55459,N58145,N64741,NB0129,R06601,R16596,R33614,R38064,R38383,R51912,R52651,R62603,R69798,R85916,W47106,W85875 GENE_SYMBOLS CEBPD,ABL1,IL22RA1,FOXO1,IRS2,GASK1B,FOXO1,S1PR3,DKK1,GAS1,PHKG1,CTNNB1,PPP1CA,E2F4,PRSS23-AS1,GM2A,USP4,CYBRD1,PIK3R1,IL1R1,MYC,SMARCD2,SOD2,USP53,GADD45B,ABCA1,MRPS6,WASF3,NFIL3,HACD3,ACVR2A,,PLIN2,PDE10A,SIK2,PDE6H,MYADM,NID1,FAM174C,OXR1,MT1E,COL8A1,P3H2,PDE4D,ELK3,,CCRL2,MT1L,,MT1G,SNAI2,NFE2,INSIG1,MT1B,MT1H,,,FYN,TGFBR3,MT1F,LHFPL6,SNAI2,,MT1M,MT1B,,LIFR,ZFP36,SST,NPTXR,COL6A3,,,SMIM3,CHMP1B FOUNDER_NAMES