STANDARD_NAME NAKAMURA_CANCER_MICROENVIRONMENT_DN SYSTEMATIC_NAME M2427 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/NAKAMURA_CANCER_MICROENVIRONMENT_DN NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes down-regulated in pancreatic cancer cells grown in orthotopic xenograft tumors compared to those grown in vitro. DESCRIPTION_FULL To determine the influence of the microenvironment on changes in gene expression, we did microarray analysis on three variant lines of a human pancreatic cancer (FG, L3.3, and L3.6pl) with different metastatic potentials. The variant lines were grown in tissue culture in the subcutis (ectopic) or pancreas (orthotopic) of nude mice. Compared with tissue culture, the number of genes of which the expression was affected by the microenvironment was up-regulated in tumors growing in the subcutis and pancreas. In addition, highly metastatic L3.6pl cells growing in the pancreas expressed significantly higher levels of 226 genes than did the L3.3 or FG variant cells. Growth of the variant lines in the subcutis did not yield similar results, indicating that the orthotopic microenvironment significantly influences gene expression in pancreatic cancer cells. These data suggest that investigations of the functional consequence of gene expression require accounting for experimental growth conditions. PMID 17210693 GEOID AUTHORS Nakamura T,Fidler IJ,Coombes KR CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 2 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1555427_s_at,201263_at,201266_at,201475_x_at,202402_s_at,202481_at,202533_s_at,202705_at,202737_s_at,202870_s_at,203145_at,203209_at,203405_at,203968_s_at,204023_at,204033_at,204092_s_at,204744_s_at,205047_s_at,205194_at,205401_at,206085_s_at,207528_s_at,208079_s_at,208511_at,208778_s_at,208969_at,209408_at,209545_s_at,209642_at,213008_at,213671_s_at,214452_at,214794_at,216052_x_at,217080_s_at,217829_s_at,217853_at,218073_s_at,218516_s_at,220181_x_at,221059_s_at,223062_s_at,225285_at,225300_at,225687_at,226001_at,226517_at,226661_at,228400_at GENE_SYMBOLS SYNCRIP,TARS1,TXNRD1,MARS1,CARS1,DHRS3,DHFR,CCNB2,LSM4,CDC20,SPAG5,RFC5,PSMG1,CDC6,RFC4,TRIP13,AURKA,IARS1,ASNS,PSPH,AGPS,CTH,SLC7A11,AURKA,PTTG3P,TCP1,NDUFA9,KIF2C,RIPK2,BUB1,FANCI,MARS1,BCAT1,PA2G4,ARTN,HOMER2,USP39,TNS3,NDC1,BPNT2,SLC30A5,COTL1,PSAT1,BCAT1,KNSTRN,FAM83D,KLHL5,BCAT1,CDCA2,SHROOM3 FOUNDER_NAMES