STANDARD_NAME NAKAMURA_METASTASIS_MODEL_DN SYSTEMATIC_NAME M15940 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/NAKAMURA_METASTASIS_MODEL_DN NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Bottom genes down-regulated in subcutaneous tumors from highly metastatic pancreatic cancer cells. DESCRIPTION_FULL To determine the influence of the microenvironment on changes in gene expression, we did microarray analysis on three variant lines of a human pancreatic cancer (FG, L3.3, and L3.6pl) with different metastatic potentials. The variant lines were grown in tissue culture in the subcutis (ectopic) or pancreas (orthotopic) of nude mice. Compared with tissue culture, the number of genes of which the expression was affected by the microenvironment was up-regulated in tumors growing in the subcutis and pancreas. In addition, highly metastatic L3.6pl cells growing in the pancreas expressed significantly higher levels of 226 genes than did the L3.3 or FG variant cells. Growth of the variant lines in the subcutis did not yield similar results, indicating that the orthotopic microenvironment significantly influences gene expression in pancreatic cancer cells. These data suggest that investigations of the functional consequence of gene expression require accounting for experimental growth conditions. PMID 17210693 GEOID AUTHORS Nakamura T,Fidler IJ,Coombes KR CONTRIBUTOR Jessica Robertson CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 3S FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1556592_at,1557905_s_at,1559116_s_at,1559331_x_at,201860_s_at,202022_at,202068_s_at,202345_s_at,203409_at,204348_s_at,204469_at,204673_at,204710_s_at,205014_at,205201_at,207360_s_at,207467_x_at,208908_s_at,209651_at,210139_s_at,210355_at,210664_s_at,210916_s_at,212268_at,213603_s_at,217561_at,217767_at,218644_at,218717_s_at,219093_at,219511_s_at,219911_s_at,220974_x_at,221524_s_at,222238_s_at,222688_at,223407_at,223432_at,223482_at,224967_at,226120_at,229638_at,231776_at,232231_at,234725_s_at,237311_at,238460_at,39248_at,39249_at,91703_at GENE_SYMBOLS ,CD44,TMEM167B-DT,,PLAT,ALDOC,LDLR,FABP5,DDB2,AK4,PTPRZ1,MUC2,WIPI2,FGFBP1,GLI3,NTSR1,CAST,CAST,TGFB1I1,PMP22,PTHLH,TFPI,CD44,SERPINB1,RAC2,CALCA,C3,PLEK2,P3H2,PID1,SNCAIP,SLCO4A1,SFXN3,RRAGD,POLM,ACER3,ENKD1,OSBP2,TMEM120A,UGCG,TTC8,IRX3,EOMES,RUNX2,SEMA4B,,FAM83A,AQP3,AQP3,EHBP1L1 FOUNDER_NAMES