STANDARD_NAME NAKAYA_PBMC_FLUAD_MALE_AGE_14_27YO_1D_POSTBOOST_VS_0D_PREIMM_MF59_ADJUVANTED_1DY_ATIV_UP SYSTEMATIC_NAME M40897 COLLECTION C7:VAX MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/NAKAYA_PBMC_FLUAD_MALE_AGE_14_27YO_1D_POSTBOOST_VS_0D_PREIMM_MF59_ADJUVANTED_1DY_ATIV_UP NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes up-regulated in peripheral blood mononuclear cell 1d postboost vs 0d pre-imm in children (14-27m) (MF59-adjuvanted) after exposure to Fluad , time point 1D. Comment: ATIV DESCRIPTION_FULL The dynamics and molecular mechanisms underlying vaccine immunity in early childhood remain poorly understood. Here we applied systems approaches to investigate the innate and adaptive responses to trivalent inactivated influenza vaccine (TIV) and MF59-adjuvanted TIV (ATIV) in 90 14- to 24-mo-old healthy children. MF59 enhanced the magnitude and kinetics of serum antibody titers following vaccination, and induced a greater frequency of vaccine specific, multicytokine-producing CD4(+) T cells. Compared with transcriptional responses to TIV vaccination previously reported in adults, responses to TIV in infants were markedly attenuated, limited to genes regulating antiviral and antigen presentation pathways, and observed only in a subset of vaccinees. In contrast, transcriptional responses to ATIV boost were more homogenous and robust. Interestingly, a day 1 gene signature characteristic of the innate response (antiviral IFN genes, dendritic cell, and monocyte responses) correlated with hemagglutination at day 28. These findings demonstrate that MF59 enhances the magnitude, kinetics, and consistency of the innate and adaptive response to vaccination with the seasonal influenza vaccine during early childhood, and identify potential molecular correlates of antibody responses. PMID 26755593 GEOID AUTHORS Nakaya HI,Clutterbuck E,Kazmin D,Wang L,Cortese M,Bosinger SE,Patel NB,Zak DE,Aderem A,Dong T,Del Giudice G,Rappuoli R,Cerundolo V,Pollard AJ,Pulendran B,Siegrist CA CONTRIBUTOR HIPC SIGNATURES CONTRIBUTOR_ORG NIAID/HIPC SIGNATURES EXACT_SOURCE Fig S5 (left) FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1519690113/-/DCSupplemental SOURCE_MEMBERS ANKRD22,ATF3,BATF2,CALHM6,CD274,CEACAM1,CXCL10,EFCAB2,EPSTI1,ETV7,FBXO6,FCGR1A,FCGR1B,GBP1,GBP4,GBP5,GK,ICAM1,IDO1,IFI35,IFI44L,IL15,KREMEN1,LAMP3,LAP3,LHFPL2,MYOF,OAS3,P2RY14,PSTPIP2,RSAD2,SCARF1,SERPING1,SMCO4,SORT1,STAT1,TFEC,TYMP,VAMP5,WARS GENE_SYMBOLS ANKRD22,ATF3,BATF2,CALHM6,CD274,CEACAM1,CXCL10,EFCAB2,EPSTI1,ETV7,FBXO6,FCGR1A,FCGR1BP,GBP1,GBP4,GBP5,GK,ICAM1,IDO1,IFI35,IFI44L,IL15,KREMEN1,LAMP3,LAP3,LHFPL2,MYOF,OAS3,P2RY14,PSTPIP2,RSAD2,SCARF1,SERPING1,SMCO4,SORT1,STAT1,TFEC,TYMP,VAMP5,WARS1 FOUNDER_NAMES