STANDARD_NAME OSWALD_HEMATOPOIETIC_STEM_CELL_IN_COLLAGEN_GEL_DN SYSTEMATIC_NAME M7089 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/OSWALD_HEMATOPOIETIC_STEM_CELL_IN_COLLAGEN_GEL_DN NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes down-regulated in hematopoietic stem cells (HSC, CD34+ [GeneID=947]) cultured in a three-dimentional collagen gel compared to the cells grown in suspension. DESCRIPTION_FULL CD34+ hematopoietic stem/progenitor cells (HSCs) reside in the bone marrow in close proximity to the endosteal bone surface, surrounded by osteoblasts, stromal cells, and various extracellular matrix molecules. We used a bioartificial matrix of fibrillar collagen I, the major matrix component of bone, as a scaffold for ex vivo expansion of HSCs. CD34+ HSCs were isolated from umbilical cord blood and cultivated within reconstituted collagen I fibrils in the presence of fms-like tyrosine kinase-3 ligand, stem cell factor, and interleukin (IL)-3. After 7 days of culture, the cell number, number of colony-forming units (CFU-C), and gene-expression profile of the cultured cells were assessed. Although the total expansion factor of CD34+ cells was slightly lower when cells were cultivated in the collagen I gel, the frequency of CFU-C was greater than in control suspension cultures. Gene-expression analysis with microarray chip technology revealed the upregulation of more than 50 genes in the presence of collagen I. Among these, genes for several growth factors, cytokines, and chemokines (e.g., IL-8 and macrophage inhibitory protein 1alpha) could be confirmed using quantitative polymerase chain reaction. Furthermore, greater expression levels of the negative cell-cycle regulator BTG2/TIS21 and an inhibitor of the mitogen-activated protein kinase pathway, DUSP2, underline the regulatory role of the extracellular matrix. Together, these data show that the expansion of CD34+ cord blood cells in a culture system containing a three-dimensional collagen I matrix induces a qualitative change in the gene-expression profile of cultivated HSCs. PMID 16166251 GEOID GSE3003 AUTHORS Oswald J,Steudel C,Salchert K,Joergensen B,Thiede C,Ehninger G,Werner C,Bornhäuser M CONTRIBUTOR Leona Saunders CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE supplementdown.pdf FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1729_at,200727_s_at,200800_s_at,200841_s_at,200923_at,201005_at,201010_s_at,201110_s_at,201123_s_at,201382_at,201425_at,201445_at,201548_s_at,201747_s_at,201777_s_at,201793_x_at,201848_s_at,201849_at,201861_s_at,201888_s_at,201927_s_at,201996_s_at,202022_at,202131_s_at,202203_s_at,202283_at,202358_s_at,202364_at,202372_at,202397_at,202498_s_at,202499_s_at,202581_at,202631_s_at,202686_s_at,202733_at,202773_s_at,202855_s_at,202856_s_at,202960_s_at,202973_x_at,202996_at,203361_s_at,203397_s_at,203439_s_at,203487_s_at,203725_at,203989_x_at,204096_s_at,204106_at,204116_at,204243_at,204314_s_at,204316_at,204319_s_at,204326_x_at,204351_at,204415_at,204523_at,204524_at,204569_at,204671_s_at,204674_at,204745_x_at,204960_at,204999_s_at,205047_s_at,205092_x_at,205160_at,205370_x_at,205371_s_at,205372_at,205382_s_at,205407_at,205497_at,205511_at,205584_at,205612_at,205619_s_at,205669_at,205777_at,205801_s_at,205837_s_at,205839_s_at,205846_at,205871_at,205898_at,205967_at,206108_s_at,206278_at,206390_x_at,206461_x_at,206507_at,206519_x_at,206789_s_at,206826_at,206925_at,206950_at,206978_at,207076_s_at,207102_at,207186_s_at,207231_at,207350_s_at,207522_s_at,207543_s_at,207686_s_at,207777_s_at,207815_at,207920_x_at,207996_s_at,208010_s_at,208119_s_at,208121_s_at,208328_s_at,208456_s_at,208478_s_at,208581_x_at,208651_x_at,208710_s_at,208712_at,208721_s_at,208876_s_at,208937_s_at,209006_s_at,209007_s_at,209077_at,209146_at,209290_s_at,209328_x_at,209583_s_at,209616_s_at,209754_s_at,209811_at,209822_s_at,209875_s_at,209920_at,209997_x_at,210001_s_at,210050_at,210195_s_at,210220_at,210279_at,210306_at,210312_s_at,210470_x_at,210524_x_at,210854_x_at,210889_s_at,210904_s_at,211074_at,211077_s_at,211081_s_at,211298_s_at,211456_x_at,211597_s_at,211665_s_at,211796_s_at,211814_s_at,211947_s_at,212033_at,212070_at,212077_at,212185_x_at,212209_at,212230_at,212286_at,212366_at,212384_at,212509_s_at,212592_at,212620_at,212647_at,212760_at,212779_at,212837_at,212859_x_at,213221_s_at,213286_at,213313_at,213341_at,213418_at,213470_s_at,213489_at,213548_s_at,213554_s_at,213650_at,213926_s_at,213930_at,213998_s_at,214012_at,214045_at,214059_at,214097_at,214132_at,214146_s_at,214202_at,214500_at,214590_s_at,214627_at,214670_at,214786_at,214820_at,214847_s_at,214866_at,214877_at,214934_at,214953_s_at,215046_at,215092_s_at,215128_at,215220_s_at,215343_at,215780_s_at,216191_s_at,216336_x_at,216505_x_at,216713_at,216722_at,216823_at,216858_x_at,216902_s_at,217164_at,217234_s_at,217408_at,217509_x_at,217552_x_at,217644_s_at,217673_x_at,217704_x_at,217777_s_at,217867_x_at,217960_s_at,217971_at,218017_s_at,218297_at,218312_s_at,218318_s_at,218370_s_at,218404_at,218490_s_at,218521_s_at,218645_at,218712_at,218816_at,218851_s_at,219125_s_at,219232_s_at,219255_x_at,219280_at,219327_s_at,219351_at,219375_at,219376_at,219410_at,219437_s_at,219506_at,219598_s_at,219618_at,219670_at,219762_s_at,219800_s_at,219822_at,219878_s_at,219880_at,219892_at,219966_x_at,220033_at,220159_at,220169_at,220235_s_at,220368_s_at,220558_x_at,220564_at,220669_at,220702_at,220748_s_at,220770_s_at,220785_at,220800_s_at,221155_x_at,221419_s_at,221428_s_at,221524_s_at,221695_s_at,221699_s_at,221732_at,221748_s_at,221765_at,221788_at,221892_at,221942_s_at,221986_s_at,222142_at,222156_x_at,222163_s_at,222320_at,32069_at,33148_at,34449_at,48808_at,55065_at,55662_at,61732_r_at,65438_at,AFFX-HUMRGE/M10098_5_at,AFFX-M27830_5_at,AFFX-r2-Hs18SrRNA-5_at,AFFX-r2-Hs28SrRNA-5_at GENE_SYMBOLS TRADD,ACTR2,HSPA1A,EPRS1,LGALS3BP,CD9,TXNIP,THBS1,EIF5A,CACYBP,ALDH2,CNN3,KDM5B,SAFB,EFCAB14,SMG7,BNIP3,BNIP3,LRRFIP1,IL13RA1,PKP4,SPEN,ALDOC,RIOK3,AMFR,SERPINF1,SNX19,MXI1,RAB3GAP2,NUTF2,SLC2A3,SLC2A3,HSPA1B,APPBP2,AXL,P4HA2,SFSWAP,SLC16A3,SLC16A3,MMUT,FAM13A,POLD4,MYCBP,GALNT3,STC2,ARMC8,GADD45A,F2R,ELL,TESK1,IL2RG,RLF,CREB1,RGS10,RGS10,MT1X,S100P,IFI6,ZNF140,PDPK1,CILK1,ANKRD6,IRAG2,MT1G,PTPRCAP,ATF5,ASNS,ZBTB1,PEX11A,DBT,DBT,PLAG1,CFD,RECK,ZNF175,GABPB1-IT1,ALG13,MMRN1,MEOX1,NCAM2,DUSP9,RASGRP3,GYPA,TSPOAP1,PTPRB,PLG,CX3CR1,H4C3,SRSF6,PTAFR,PF4,MT1H,ZSCAN12,SIGLEC6,POU2F1,PMP2,ST8SIA4,SCN9A,CCR2,ASS1,AKR1D1,BPTF,DZIP3,VAMP4,ATP2A3,P4HA1,CASP8,SP140,PF4V1,ZFX,LDLRAD4,PTPN22,ZNF93,PTPRO,MEF2A,RRAS2,BAX,MT1X,CD24,AP3D1,CCND1,ANAPC5,PAK2,ID1,RSRP1,RSRP1,TXN2,MSMO1,NFIB,HIGD2A,CD200,CES1,TMPO,CASP2,VLDLR,SPP1,BMPR2,PCM1,SOCS1,TPI1,PSG1,FZD2,GPR18,L3MBTL1,IFT20,NONO,MT1F,SLC6A8,FCGR2B,IL13RA1,,TLK1,MAP4K5,ALB,MT1H,HOPX,SOS2,TRBC2,CCNE2,PRRC2C,RBM25,ADGRG1,CALD1,MT2A,MED13L,PLPP3,ANKRD12,ZNF292,DDX39B,MXRA7,JCHAIN,ZNF609,RRAS,UBR2,BLTP1,ABRAXAS2,MT1E,SIK2,ZFR,RABGAP1,FEM1C,HSPA6,HNRNPH1,MAPRE2,CDV3,CDV3,GOLGA8A,AGFG1,ATG12,DDX17,ERAP1,LIAS,IFI44,RPS21,,PPBP,PGGT1B,MACROH2A1,UBE2D1,EPX,ZKSCAN1,MAP3K1,BRWD1,GPSM3,PLAUR,CDKAL1,ATP9B,APP,KANSL1L,NFAT5,NOPCHAP1,TPR,CCDC88C,SET,TRDC,,RPS10P5,KRIT1,VENTXP1,RPS3AP5,,RRN3,,EZR,MRPS18B,GRIK5,CR1,SOS2,,,HACD3,BACE2,TOMM22,LAMTOR3,HGSNAT,MINDY3,ZSCAN18,NLK,S100PBP,SNX10,ZNF302,UBE2W,ZNF277,C1orf109,LRRC1,WDR33,SLC50A1,EGLN3,IL17RB,BRWD1,GPRC5C,TRAPPC2,CEPT1,ZNF322,TMEM45A,ANKRD11,C1orf54,RWDD1,IRAK4,BEND5,RPL36,THNSL1,MTRF1,KLF13,CTC-338M12.4,TM6SF1,BANP,CYB5RL,ABCA11P,TMEM156,LRIF1,PPP4R3A,TSPAN32,RNLS,OTUD4,,ZNF580,FAM200C,UTS2,TMOD3,,,TBL1XR1,RRAGD,MAP3K2,DDX50,CANT1,TNS1,UGCG,PGM3,H6PD,GUCY1A1,KLHL24,CYLD,CCPG1,SPATA5L1,,N4BP1,ZFR,CASP2,DHFR,MARK4,ARMH3,IFT74,MEAK7,MIR3648-1,,MIR3648-1, FOUNDER_NAMES