STANDARD_NAME	OXFORD_RALA_OR_RALB_TARGETS_DN
SYSTEMATIC_NAME	M1236
COLLECTION	ARCHIVED:C2_CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/OXFORD_RALA_OR_RALB_TARGETS_DN
NAMESPACE	UniGene_ID
DESCRIPTION_BRIEF	Genes down-regulated after knockdown of RALA or RALB [GeneiD=5898;5899], which were also differentially expressed in bladder cancer compared to normal bladder urothelium tissue.
DESCRIPTION_FULL	Although the monomeric GTPases RalA and RalB have been shown to regulate a variety of transcription factors, little is known regarding the differences or similarities in transcriptional programs regulated by RalA compared to RalB. Further, the association of these transcriptional pathways to human carcinogenesis and progression remains unclear. Here, we studied the role of RalA and/or RalB in transcriptional regulation by combining short interfering RNA depletion of Ral with gene expression profiling via microarray in the human bladder cancer cell line, UMUC-3. A large number of genes were found to be similarly modulated in cells with RalA and RalB depletion, suggesting that RalA and RalB impinge on overlapping transcriptional signaling pathways. However, smaller sets of genes were modulated by depletion of RalA or RalB, indicating that these closely related proteins also regulate nonoverlapping transcriptional pathways. Computational analysis of upstream sequences of genes modulated by Ral depletion identified Ras-responsive element-binding protein (RREB)-1, as a putative Ral transcriptional target, which we verified experimentally. Importantly, as a group, Ral-regulated probe sets identified here were disproportionally represented among those differentially expressed as a function of human bladder transformation. Taken together, these data strongly suggest that Ral family members mediate both common and specific transcriptional programs that are associated with human cancer and identify RREB-1 as a novel transcriptional effector of Ral.
PMID	17496927
GEOID	
AUTHORS	Oxford G,Smith SC,Hampton G,Theodorescu D
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 4BS
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	Hs.118400,Hs.133892,Hs.151220,Hs.210995,Hs.273330,Hs.2785,Hs.303090,Hs.406515,Hs.433213,Hs.435714,Hs.436657,Hs.465929,Hs.471381,Hs.485518,Hs.490203,Hs.4998,Hs.500761,Hs.503163,Hs.518250,Hs.529989,Hs.540696,Hs.556244,Hs.591588,Hs.632285,Hs.647069,Hs.73793
GENE_SYMBOLS	,,,,,,,,,,,,,,,,,,,,,,,,,
FOUNDER_NAMES