STANDARD_NAME	PIEPOLI_LGI1_TARGETS_UP
SYSTEMATIC_NAME	M15008
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/PIEPOLI_LGI1_TARGETS_UP
NAMESPACE	AFFY_HG_U95
DESCRIPTION_BRIEF	Up-regulated genes in U87 cells (glioblastoma multiforme, GBM) engineered to stably express LGI1 [GeneID=9211].
DESCRIPTION_FULL	Disruptions of LGI1 in glioblastoma (GBM) cell lines and LGI1 mutations in families with autosomal dominant epilepsy imply a role for LGI1 in glial cells as well as in neurons. Although we and others could not find LGI1 mutations in malignant gliomas, our initial studies appeared to support the idea that LGI1 is poorly expressed or absent in these tumors. Microarray data suggested that LGI1 could be involved in the control of matrix metalloproteinases, and we found that tumors derived from U87 glioblastoma cells overexpressing LGI1 were less aggressive than U87 control tumors. To our surprise, we observed that LGI1 expression after differentiation of murine neural stem cells was robust in neurons but negligible in glial cells, in agreement with immunohistochemistry studies on rodent brain. This observation could suggest that the variable levels of LGI1 expression in gliomas reflect the presence of neurons entrapped within the tumor. To test this hypothesis, we investigated LGI1 expression in parallel with expression of the neuronal marker NEF3 by real-time PCR on 30 malignant gliomas. Results showed a strong, positive correlation between the expression levels of these two genes (P < 0.0001). Thus, our data confirm that LGI1 is involved in cell-matrix interactions but suggest that its expression is not relevant in glial cells, implying that its role as a tumor suppressor in gliomas should be reconsidered.
PMID	16533756
GEOID	
AUTHORS	Piepoli T,Jakupoglu C,Gu W,Lualdi E,Suarez-Merino B,Poliani PL,Cattaneo MG,Ortino B,Goplen D,Wang J,Mola R,Inverardi F,Frassoni C,Bjerkvig R,Steinlein O,Vicentini LM,Brüstle O,Finocchiaro G
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 1BS
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	1035_g_at,208_at,31498_f_at,31599_f_at,31954_f_at,31960_f_at,33047_at,33517_f_at,33671_f_at,33680_f_at,33714_at,34564_at,34575_f_at,34965_at,36232_at,37611_at,38263_at,38627_at,38886_i_at,39592_r_at,41504_s_at,41505_r_at,41722_at
GENE_SYMBOLS	TIMP3,CTNNA2,GAGE1,MAGEA3,GAGE1,GAGE1,BCL2L11,MAGEA3,GAGE1,GAGE1,HMGB3,EPHA5,MAGEA5P,CST7,FGF13,TNFRSF11B,RYR2,HLF,DIRAS3,FGL2,MAF,MAF,NNT
FOUNDER_NAMES