STANDARD_NAME REN_BOUND_BY_E2F SYSTEMATIC_NAME M4493 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/REN_BOUND_BY_E2F NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes whose promoters were bound by E2F1 and E2F4 [GeneID=1869;1874] in the primary fibroblasts WI-38, by ChIP on chip assay. DESCRIPTION_FULL The E2F transcription factor family is known to play a key role in the timely expression of genes required for cell cycle progression and proliferation, but only a few E2F target genes have been identified. We explored the possibility that E2F regulators play a broader role by identifying additional genes bound by E2F in living human cells. A protocol was developed to identify genomic binding sites for DNA-binding factors in mammalian cells that combines immunoprecipitation of cross-linked protein-DNA complexes with DNA microarray analysis. Among approximately 1200 genes expressed during cell cycle entry, we found that the promoters of 127 were bound by the E2F4 transcription factor in primary fibroblasts. A subset of these targets was also bound by E2F1. Most previously identified target genes known to have roles in DNA replication and cell cycle control and represented on the microarray were confirmed by this analysis. We also identified a remarkable cadre of genes with no previous connection to E2F regulation, including genes that encode components of the DNA damage checkpoint and repair pathways, as well as factors involved in chromatin assembly/condensation, chromosome segregation, and the mitotic spindle checkpoint. Our data indicate that E2F directly links cell cycle progression with the coordinate regulation of genes essential for both the synthesis of DNA as well as its surveillance. PMID 11799067 GEOID AUTHORS Ren B,Cam H,Takahashi Y,Volkert T,Terragni J,Young RA,Dynlacht BD CONTRIBUTOR Yujin Hoshida CONTRIBUTOR_ORG Broad Institute EXACT_SOURCE Fig. 3 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS ASK,BARD1,BRD2,BUB3,CBX5,CCNA2,CDC2,CDC25A,CDC6,CDK2,CENPA,CENPE,CHEK1,CKS1,CSTF1,DUT,E2F2,E2F3,FEN1,H2AFL,H2AFO,H2AFX,H2AFZ,H2BFQ,H3FB,HEC,HMG1,HMG4,MAD2L1,MCM3,MCM5,MCM6,MLH1,MSH2,NAP1L4,ORC1L,PCNA,POLA,POLA2,POLD1,PRIM2A,PRKDC,PTTG1,RAD51,RAD54L,RB1,RBL1,RFC2,RFC3,RFC4,RPA3,RRM1,SMARCA3,SMC2L1,SMC4L1,SUPT4H1,TK1,TOP2A,TP53,TTK,UMPS,UNG GENE_SYMBOLS DBF4,BARD1,BRD2,BUB3,CBX5,CCNA2,CDK1,CDC25A,CDC6,CDK2,CENPA,CENPE,CHEK1,CKS1BP7,CSTF1,DUT,E2F2,E2F3,FEN1,H2AC6,H2AC18,H2AX,H2AZ1,H2BC21,H3C4,NDC80,HMGB1,HMGB3,MAD2L1,MCM3,MCM5,MCM6,MLH1,MSH2,NAP1L4,ORC1,PCNA,POLA1,POLA2,POLD1,PRIM2,PRKDC,PTTG1,RAD51,RAD54L,RB1,RBL1,RFC2,RFC3,RFC4,RPA3,RRM1,HLTF,SMC2,SMC4,SUPT4H1,TK1,TOP2A,TP53,TTK,UMPS,UNG FOUNDER_NAMES