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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="RIZ_ERYTHROID_DIFFERENTIATION_12HR" SYSTEMATIC_NAME="M4389" HISTORICAL_NAMES="" PMID="17213805" AUTHORS="Riz I,Akimov SS,Eaker SS,Baxter KK,Lee HJ,Mariño-Ramírez L,Landsman D,Hawley TS,Hawley RG" GEOID="" EXACT_SOURCE="Table 7S" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_MG_U74" CONTRIBUTOR="Leona Saunders" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Selected genes down-regulated in the TLX1 [GeneID=3195] Tet On iEBHX15-4 cells (pro-erythroblasts) at 12 h time point." DESCRIPTION_FULL="Aberrant expression of the human homeobox-containing proto-oncogene TLX1/HOX11 inhibits hematopoietic differentiation programs in a number of murine model systems. Here, we report the establishment of a murine erythroid progenitor cell line, iEBHX1S-4, developmentally arrested by regulatable TLX1 expression. Extinction of TLX1 expression released the iEBHX1S-4 differentiation block, allowing erythropoietin-dependent acquisition of erythroid markers and hemoglobin synthesis. Coordinated activation of erythroid transcriptional networks integrated by the acetyltransferase co-activator CREB-binding protein (CBP) was suggested by bioinformatic analysis of the upstream regulatory regions of several conditionally induced iEBHX1S-4 gene sets. In accord with this notion, CBP-associated acetylation of GATA-1, an essential regulator of erythroid differentiation, increased concomitantly with TLX1 downregulation. Coimmunoprecipitation experiments and glutathione-S-transferase pull-down assays revealed that TLX1 directly binds to CBP, and confocal laser microscopy demonstrated that the two proteins partially colocalize at intranuclear sites in iEBHX1S-4 cells. Notably, the distribution of CBP in conditionally blocked iEBHX1S-4 cells partially overlapped with chromatin marked by a repressive histone methylation pattern, and downregulation of TLX1 coincided with exit of CBP from these heterochromatic regions. Thus, we propose that TLX1-mediated differentiation arrest may be achieved in part through a mechanism that involves redirection of CBP and/or its sequestration in repressive chromatin domains." TAGS="" MEMBERS="100130_at,100962_at,101797_at,102984_g_at,103925_at,104233_at,104376_at,104381_at,104462_at,104698_at,160901_at,161113_at,161383_r_at,161390_r_at,161468_f_at,161699_i_at,161994_f_at,92186_at,92667_at,92910_at,92930_at,92974_at,93142_at,93350_f_at,93528_s_at,93601_at,93657_at,93728_at,94687_at,94811_s_at,95315_at,96580_at,96940_at,96993_at,97193_at,97497_at,97747_r_at,98083_at,98340_at,98579_at,98838_at,99665_at,99875_at,99901_at,99914_at" MEMBERS_SYMBOLIZED="AR,ARNT2,ARX,BACH1,CAVIN1,DLX5,EGR1,ESR1,FOS,FOXB1,GATA6,HAND2,HDAC5,HIC1,HOXA4,HR,JUN,KLF6,KLF9,MLLT3,NAB2,NDN,NOTCH1,NR1H3,PAX9,PBX3,SATB1,SMAD1,SPIB,TEAD1,TEAD2,TFCP2L1,TSC22D1,ZFP14,ZFP37,ZNF22" MEMBERS_EZID="10014,10062,1316,170302,1749,1958,2099,2353,2627,27023,284119,29842,3090,3201,367,3725,4086,4300,4665,4692,4851,5083,5090,55806,571,57677,6304,6689,687,7003,7539,7570,8463,8848,9464,9915" MEMBERS_MAPPING="100130_at,JUN,3725|100962_at,NAB2,4665|101797_at,null,null|102984_g_at,SMAD1,4086|103925_at,MLLT3,4300|104233_at,TEAD1,7003|104376_at,HDAC5,10014|104381_at,NR1H3,10062|104462_at,HIC1,3090|104698_at,GATA6,2627|160901_at,FOS,2353|161113_at,ESR1,2099|161383_r_at,null,null|161390_r_at,null,null|161468_f_at,null,null|161699_i_at,null,null|161994_f_at,null,null|92186_at,ARX,170302|92667_at,AR,367|92910_at,ARNT2,9915|92930_at,DLX5,1749|92974_at,ZFP37,7539|93142_at,BACH1,571|93350_f_at,ZNF22,7570|93528_s_at,KLF9,687|93601_at,null,null|93657_at,SPIB,6689|93728_at,TSC22D1,8848|94687_at,FOXB1,27023|94811_s_at,NDN,4692|95315_at,ZFP14,57677|96580_at,PBX3,5090|96940_at,TEAD2,8463|96993_at,null,null|97193_at,TFCP2L1,29842|97497_at,NOTCH1,4851|97747_r_at,HOXA4,3201|98083_at,KLF6,1316|98340_at,null,null|98579_at,EGR1,1958|98838_at,PAX9,5083|99665_at,SATB1,6304|99875_at,HR,55806|99901_at,CAVIN1,284119|99914_at,HAND2,9464" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Mus musculus" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
