STANDARD_NAME	RIZ_ERYTHROID_DIFFERENTIATION_6HR
SYSTEMATIC_NAME	M1854
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/RIZ_ERYTHROID_DIFFERENTIATION_6HR
NAMESPACE	AFFY_MG_U74
DESCRIPTION_BRIEF	Selected genes down-regulated in the TLX1 [GeneID=3195] Tet On iEBHX15-4 cells (pro-erythroblasts) at 6 h time point.
DESCRIPTION_FULL	Aberrant expression of the human homeobox-containing proto-oncogene TLX1/HOX11 inhibits hematopoietic differentiation programs in a number of murine model systems. Here, we report the establishment of a murine erythroid progenitor cell line, iEBHX1S-4, developmentally arrested by regulatable TLX1 expression. Extinction of TLX1 expression released the iEBHX1S-4 differentiation block, allowing erythropoietin-dependent acquisition of erythroid markers and hemoglobin synthesis. Coordinated activation of erythroid transcriptional networks integrated by the acetyltransferase co-activator CREB-binding protein (CBP) was suggested by bioinformatic analysis of the upstream regulatory regions of several conditionally induced iEBHX1S-4 gene sets. In accord with this notion, CBP-associated acetylation of GATA-1, an essential regulator of erythroid differentiation, increased concomitantly with TLX1 downregulation. Coimmunoprecipitation experiments and glutathione-S-transferase pull-down assays revealed that TLX1 directly binds to CBP, and confocal laser microscopy demonstrated that the two proteins partially colocalize at intranuclear sites in iEBHX1S-4 cells. Notably, the distribution of CBP in conditionally blocked iEBHX1S-4 cells partially overlapped with chromatin marked by a repressive histone methylation pattern, and downregulation of TLX1 coincided with exit of CBP from these heterochromatic regions. Thus, we propose that TLX1-mediated differentiation arrest may be achieved in part through a mechanism that involves redirection of CBP and/or its sequestration in repressive chromatin domains.
PMID	17213805
GEOID	
AUTHORS	Riz I,Akimov SS,Eaker SS,Baxter KK,Lee HJ,Mariño-Ramírez L,Landsman D,Hawley TS,Hawley RG
CONTRIBUTOR	Leona Saunders
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 6S
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	101162_at,101396_at,101904_at,102048_at,102087_at,102579_f_at,102668_at,102955_at,103283_at,104240_at,104590_at,160604_at,160731_at,161418_r_at,161584_r_at,162314_at,92248_at,92255_at,92344_at,92535_at,92614_at,92717_at,92883_at,92891_f_at,92894_s_at,92896_s_at,92902_at,92999_at,93230_at,93371_at,93418_g_at,93613_at,94100_s_at,94636_at,94710_g_at,95391_at,95584_at,95968_at,97357_at,98780_at,99058_at,99427_at,99432_at,99965_at
GENE_SYMBOLS	MYF5,HNF1B,,ANKRD1,HOXA3,HOXA6,PPARA,NFIL3,ELF5,CUX1,MEF2C,FOXC1,RAB25,,,,NR4A2,HOXB4,HLTF,EBF1,ID3,NEUROD1,RAB1A,HOXC9,NFIA,SIM2,MYBL1,SIX4,NEUROG1,BNC1,MEF2B,SMAD3,TRPC4,HOXA13,GCM2,VAX1,DPPA2,,MEF2C,HOXB3,HMGA2,HOXD12,CLIP2,VDR
FOUNDER_NAMES