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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="RIZ_ERYTHROID_DIFFERENTIATION_6HR" SYSTEMATIC_NAME="M1854" HISTORICAL_NAMES="" PMID="17213805" AUTHORS="Riz I,Akimov SS,Eaker SS,Baxter KK,Lee HJ,Mariño-Ramírez L,Landsman D,Hawley TS,Hawley RG" GEOID="" EXACT_SOURCE="Table 6S" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_MG_U74" CONTRIBUTOR="Leona Saunders" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Selected genes down-regulated in the TLX1 [GeneID=3195] Tet On iEBHX15-4 cells (pro-erythroblasts) at 6 h time point." DESCRIPTION_FULL="Aberrant expression of the human homeobox-containing proto-oncogene TLX1/HOX11 inhibits hematopoietic differentiation programs in a number of murine model systems. Here, we report the establishment of a murine erythroid progenitor cell line, iEBHX1S-4, developmentally arrested by regulatable TLX1 expression. Extinction of TLX1 expression released the iEBHX1S-4 differentiation block, allowing erythropoietin-dependent acquisition of erythroid markers and hemoglobin synthesis. Coordinated activation of erythroid transcriptional networks integrated by the acetyltransferase co-activator CREB-binding protein (CBP) was suggested by bioinformatic analysis of the upstream regulatory regions of several conditionally induced iEBHX1S-4 gene sets. In accord with this notion, CBP-associated acetylation of GATA-1, an essential regulator of erythroid differentiation, increased concomitantly with TLX1 downregulation. Coimmunoprecipitation experiments and glutathione-S-transferase pull-down assays revealed that TLX1 directly binds to CBP, and confocal laser microscopy demonstrated that the two proteins partially colocalize at intranuclear sites in iEBHX1S-4 cells. Notably, the distribution of CBP in conditionally blocked iEBHX1S-4 cells partially overlapped with chromatin marked by a repressive histone methylation pattern, and downregulation of TLX1 coincided with exit of CBP from these heterochromatic regions. Thus, we propose that TLX1-mediated differentiation arrest may be achieved in part through a mechanism that involves redirection of CBP and/or its sequestration in repressive chromatin domains." TAGS="" MEMBERS="101162_at,101396_at,101904_at,102048_at,102087_at,102579_f_at,102668_at,102955_at,103283_at,104240_at,104590_at,160604_at,160731_at,161418_r_at,161584_r_at,162314_at,92248_at,92255_at,92344_at,92535_at,92614_at,92717_at,92883_at,92891_f_at,92894_s_at,92896_s_at,92902_at,92999_at,93230_at,93371_at,93418_g_at,93613_at,94100_s_at,94636_at,94710_g_at,95391_at,95584_at,95968_at,97357_at,98780_at,99058_at,99427_at,99432_at,99965_at" MEMBERS_SYMBOLIZED="ANKRD1,BNC1,CLIP2,CUX1,DPPA2,EBF1,ELF5,FOXC1,GCM2,HLTF,HMGA2,HNF1B,HOXA13,HOXA3,HOXA6,HOXB3,HOXB4,HOXC9,HOXD12,ID3,MEF2B,MEF2C,MYBL1,MYF5,NEUROD1,NEUROG1,NFIA,NFIL3,NR4A2,PPARA,RAB1A,RAB25,SIM2,SIX4,SMAD3,TRPC4,VAX1,VDR" MEMBERS_EZID="100271849,11023,151871,1523,1879,2001,2296,27063,3200,3203,3209,3213,3214,3225,3238,3399,4088,4208,4603,4617,4760,4762,4774,4783,4929,51804,5465,57111,5861,646,6493,6596,6928,7223,7421,7461,8091,9247" MEMBERS_MAPPING="101162_at,MYF5,4617|101396_at,HNF1B,6928|101904_at,null,null|102048_at,ANKRD1,27063|102087_at,HOXA3,3200|102579_f_at,HOXA6,3203|102668_at,PPARA,5465|102955_at,NFIL3,4783|103283_at,ELF5,2001|104240_at,CUX1,1523|104590_at,MEF2C,4208|160604_at,FOXC1,2296|160731_at,RAB25,57111|161418_r_at,null,null|161584_r_at,null,null|162314_at,null,null|92248_at,NR4A2,4929|92255_at,HOXB4,3214|92344_at,HLTF,6596|92535_at,EBF1,1879|92614_at,ID3,3399|92717_at,NEUROD1,4760|92883_at,RAB1A,5861|92891_f_at,HOXC9,3225|92894_s_at,NFIA,4774|92896_s_at,SIM2,6493|92902_at,MYBL1,4603|92999_at,SIX4,51804|93230_at,NEUROG1,4762|93371_at,BNC1,646|93418_g_at,MEF2B,100271849|93613_at,SMAD3,4088|94100_s_at,TRPC4,7223|94636_at,HOXA13,3209|94710_g_at,GCM2,9247|95391_at,VAX1,11023|95584_at,DPPA2,151871|95968_at,null,null|97357_at,MEF2C,4208|98780_at,HOXB3,3213|99058_at,HMGA2,8091|99427_at,HOXD12,3238|99432_at,CLIP2,7461|99965_at,VDR,7421" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Mus musculus" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
