STANDARD_NAME	SHIRAISHI_PLZF_TARGETS_UP
SYSTEMATIC_NAME	M1168
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/SHIRAISHI_PLZF_TARGETS_UP
NAMESPACE	Human_RefSeq
DESCRIPTION_BRIEF	Genes up-regulated in A375 and 397 cells (melanoma) by forced expression of PLZF [GeneID=7704] off adenovirus vector.
DESCRIPTION_FULL	Promyelocytic leukemia zinc-finger (PLZF) is a transcriptional repressor and tumor suppressor. PLZF is expressed in melanocytes but not in melanoma cells, and recovery of PLZF expression markedly suppresses melanoma cell growth. Several target genes regulated by PLZF have been identified, but the precise function of PLZF remains uncertain. Here, we searched for candidate target genes of PLZF by DNA microarray analysis. Pre-B-cell leukemia transcription factor 1 (Pbx1) was one of the prominently suppressed genes. Pbx1 was highly expressed in melanoma cells, and its expression was reduced by transduction with the PLZF gene. Moreover, the growth suppression mediated by PLZF was reversed by enforced expression of Pbx1. Knockdown of Pbx1 by specific small interfering RNAs suppressed melanoma cell growth. We also found that Pbx1 binds HoxB7. Reverse transcription-polymerase chain reaction analysis demonstrated that repression of Pbx1 by PLZF reduces the expression of HoxB7 target genes, including tumor-associated neoangiogenesis factors such as basic fibroblast growth factor, angiopoietin-2 and matrix metalloprotease 9. These findings suggest that deregulation of Pbx1 expression owing to loss of PLZF expression contributes to the progression and/or pathogenesis of melanoma.
PMID	16862184
GEOID	
AUTHORS	Shiraishi K,Yamasaki K,Nanba D,Inoue H,Hanakawa Y,Shirakata Y,Hashimoto K,Higashiyama S
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 1
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	NM_001206,NM_003311,NM_003999,NM_004882,NM_005103,NM_006150,NM_006187,NM_007254,NM_014383,NM_021100
GENE_SYMBOLS	KLF9,PHLDA2,OSMR,CIR1,FEZ1,PRICKLE3,OAS3,PNKP,ZBTB32,NFS1
FOUNDER_NAMES