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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="SILIGAN_TARGETS_OF_EWS_FLI1_FUSION_DN" SYSTEMATIC_NAME="M16533" HISTORICAL_NAMES="" PMID="15735734" AUTHORS="Siligan C,Ban J,Bachmaier R,Spahn L,Kreppel M,Schaefer KL,Poremba C,Aryee DN,Kovar H" GEOID="" EXACT_SOURCE="Table 1A" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="HUMAN_GENE_SYMBOL" CONTRIBUTOR="Arthur Liberzon" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes bound by EWSR1-FLT1 [GeneID=2130;2321] fusion and down-regulated in STA-ET-7.2 cells (Ewing's sarcoma) after knockdown of EWSR1-FLT1 by RNAi." DESCRIPTION_FULL="In all, 85% of Ewing's sarcoma family tumors (ESFT), a neoplasm of unknown histogenesis, express EWS-FLI1 transcription factor gene fusions. To characterize direct target genes avoiding artificial model systems, we cloned genomic DNA from ESFT chromatin precipitating with EWS-FLI1. We now present a comprehensive list of 99 putative transcription factor targets identified, for the first time, by a hypothesis-free approach based on physical interaction. Gene-derived chromatin fragments co-precipitating with EWS-FLI1 were nonrandomly distributed over the human genome and localized predominantly to the upstream region and the first two introns of the genes. At least 20% of putative direct EWS-FLI1 targets were neural genes. One-third of genes recovered showed a significant ESFT-specific expression pattern and were found to be altered upon RNAi-mediated knockdown of EWS-FLI1. Among them, MK-STYX, encoding a MAP kinase phosphatase-like protein, was consistently expressed in ESFT. EWS-FLI1 was found to drive MK-STYX expression by binding to a single ETS binding motif within the first gene intron. MK-STYX serves as precedence for successful recovery of direct EWS-FLI1 targets from the authentic ESFT cellular context, the most relevant system to study oncogenic mechanisms for the discovery of new therapeutic targets in this disease." TAGS="" MEMBERS="ACCN1,ASNS,CDC14B,CLASP2,CNTNAP2,DLG2,GTF2E2,IDH3B,LRBA,MK-STYX,NOL5A,PCCA,PFTK1,POT1,RFC1,SLC2A5,SSR3,TBL1X" MEMBERS_SYMBOLIZED="ASIC2,ASNS,CDC14B,CDK14,CLASP2,CNTNAP2,DLG2,GTF2E2,IDH3B,LRBA,NOP56,PCCA,POT1,RFC1,SLC2A5,SSR3,STYXL1,TBL1X" MEMBERS_EZID="10528,1740,23122,25913,26047,2961,3420,40,440,5095,51657,5218,5981,6518,6747,6907,8555,987" MEMBERS_MAPPING="ACCN1,ASIC2,40|ASNS,ASNS,440|CDC14B,CDC14B,8555|CLASP2,CLASP2,23122|CNTNAP2,CNTNAP2,26047|DLG2,DLG2,1740|GTF2E2,GTF2E2,2961|IDH3B,IDH3B,3420|LRBA,LRBA,987|MK-STYX,STYXL1,51657|NOL5A,NOP56,10528|PCCA,PCCA,5095|PFTK1,CDK14,5218|POT1,POT1,25913|RFC1,RFC1,5981|SLC2A5,SLC2A5,6518|SSR3,SSR3,6747|TBL1X,TBL1X,6907" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Homo sapiens" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
