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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="SILIGAN_TARGETS_OF_EWS_FLI1_FUSION_UP" SYSTEMATIC_NAME="M9251" HISTORICAL_NAMES="" PMID="15735734" AUTHORS="Siligan C,Ban J,Bachmaier R,Spahn L,Kreppel M,Schaefer KL,Poremba C,Aryee DN,Kovar H" GEOID="" EXACT_SOURCE="Table 1B" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="HUMAN_GENE_SYMBOL" CONTRIBUTOR="Arthur Liberzon" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes bound by EWSR1-FLT1 [GeneID=2130;2321] fusion and up-regulated in STA-ET-7.2 cells (Ewing's sarcoma) after knockdown of EWSR1-FLT1 by RNAi." DESCRIPTION_FULL="In all, 85% of Ewing's sarcoma family tumors (ESFT), a neoplasm of unknown histogenesis, express EWS-FLI1 transcription factor gene fusions. To characterize direct target genes avoiding artificial model systems, we cloned genomic DNA from ESFT chromatin precipitating with EWS-FLI1. We now present a comprehensive list of 99 putative transcription factor targets identified, for the first time, by a hypothesis-free approach based on physical interaction. Gene-derived chromatin fragments co-precipitating with EWS-FLI1 were nonrandomly distributed over the human genome and localized predominantly to the upstream region and the first two introns of the genes. At least 20% of putative direct EWS-FLI1 targets were neural genes. One-third of genes recovered showed a significant ESFT-specific expression pattern and were found to be altered upon RNAi-mediated knockdown of EWS-FLI1. Among them, MK-STYX, encoding a MAP kinase phosphatase-like protein, was consistently expressed in ESFT. EWS-FLI1 was found to drive MK-STYX expression by binding to a single ETS binding motif within the first gene intron. MK-STYX serves as precedence for successful recovery of direct EWS-FLI1 targets from the authentic ESFT cellular context, the most relevant system to study oncogenic mechanisms for the discovery of new therapeutic targets in this disease." TAGS="" MEMBERS="ATP1B1,CDK7,FLJ21613,KIAA1026,KIAA1361,KIAA1432,LIMS1,LIPA,MDH2,NCAM1,NPY,PCLO,RHOBTB3,TCF12,VGF" MEMBERS_SYMBOLIZED="ATP1B1,CDK7,KAZN,LIMS1,LIPA,MDH2,NAA35,NCAM1,NPY,PCLO,RHOBTB3,RIC1,TAOK1,TCF12,VGF" MEMBERS_EZID="1022,22836,23254,27445,3987,3988,4191,4684,481,4852,57551,57589,60560,6938,7425" MEMBERS_MAPPING="ATP1B1,ATP1B1,481|CDK7,CDK7,1022|FLJ21613,NAA35,60560|KIAA1026,KAZN,23254|KIAA1361,TAOK1,57551|KIAA1432,RIC1,57589|LIMS1,LIMS1,3987|LIPA,LIPA,3988|MDH2,MDH2,4191|NCAM1,NCAM1,4684|NPY,NPY,4852|PCLO,PCLO,27445|RHOBTB3,RHOBTB3,22836|TCF12,TCF12,6938|VGF,VGF,7425" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Homo sapiens" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
