STANDARD_NAME TAKAO_RESPONSE_TO_UVB_RADIATION_UP SYSTEMATIC_NAME M73 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/TAKAO_RESPONSE_TO_UVB_RADIATION_UP NAMESPACE AFFY_HuGene DESCRIPTION_BRIEF Genes up-regulated in primary tissue culture of epidermal kerationcytes after UVB irradiation. DESCRIPTION_FULL Ultraviolet B (UVB) radiation is an important inducer of many biologic changes in skin, of which keratinocytes are a key target. To gain better insight into changes in gene expression generated in the early phase after UVB exposure, we used complementary RNA (cRNA) microarray hybridization to compare differences in mRNA expression of UVB-irradiated (single dose of 100 J/m2 broad-band UVB) and sham-irradiated primary cultured human keratinocytes. Six hours after irradiation, total RNA was isolated from keratinocytes, and cRNA was synthesized and hybridized to a GeneChip expression array (Affymetrix) consisting of 6800 genes. Based on a threshold of > twofold change, 187 genes (2.8%) were designated to be the most UVB-responsive. Surprisingly, none of these genes had been shown previously to be modulated by UVB. Conversely, several genes in the microarray that had been reported previously to be UVB- responsive by other methods showed less (< twofold) or no change. Northern blotting of seven differentially modulated genes produced results similar to those derived from microarray technology, thereby validating the accuracy of screening. Clustering based on known or likely functions indicated that among 88 upregulated genes, nine encode for cytochrome c subunits, six for ribosomal proteins, and two for regulators of apoptosis. By contrast, many of the 99 downregulated genes are involved in transcription, differentiation and transport. These findings indicate that keratinocytes respond to a single low dose of broad-band UVB irradiation by enhancing processes involved in energy production and translation, while suppressing those related to transcription, differentiation and transport. PMID 11982916 GEOID AUTHORS Takao J,Ariizumi K,Dougherty II,Cruz PD Jr CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 1 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS D42108_at,D49396_at,D86974_at,D89052_at,D89667_at,D90070_s_at,J02963_at,J04823_rna1_at,L09260_at,L09604_at,L19779_at,L19783_at,L20941_at,L32977_at,L34060_at,M14200_rna1_at,M16279_at,M17885_at,M19961_at,M22760_at,M26880_at,M28585_f_at,M29064_at,M32886_at,M37583_at,M57567_at,M58459_at,M62831_at,M63573_at,M64716_at,M68864_at,M69066_at,M69225_at,M77140_at,M80563_at,M83751_at,M86667_at,M87284_at,M90356_f_at,U05040_at,U09813_at,U12404_at,U13991_at,U14968_at,U14970_at,U14971_at,U16812_s_at,U25789_at,U28963_at,U32944_at,U35048_at,U37690_at,U39400_at,U40369_rna1_at,U41515_at,U43077_at,U47101_at,U49278_at,U49869_rna1_at,U58682_at,U63825_at,U67171_at,U73524_at,U78027_rna3_at,U78095_at,U79287_at,U80073_at,U90915_at,U91316_at,U94586_at,U96915_at,X02317_at,X13238_at,X15341_at,X15822_at,X52882_at,X59417_at,X62320_at,X69978_at,X71973_at,X77584_at,X81003_at,X85372_at,X95097_rna1_s_at,Y00433_at,Z14244_at,Z18859_rna1_at,Z21507_at,Z22548_at GENE_SYMBOLS PLCL1,PRDX3,NPIPB3,ATP6V0B,PFDN5,PMAIP1,,COX8A,SEC13,PLP2,H2AC18,PIGH,FTH1,UQCRFS1,CDH8,DBI,CD99,RPLP0,COX5B,COX5A,UBC,IFNA16,HNRNPA2B1,SRI,H2AZ1,ARF5,RPS4Y1,IER2,PPIB,RPS25,UBXN1,MSN,DST,GAL,S100A4,MANF,NAP1L1,,BTF3P13,FUBP1,ATP5MC3,RPL10A,TPP1,RPL27A,RPS5,RPS9,BAK1,RPL21,GPS2,DYNLL1,TSC22D1,POLR2L,MRPL49,SAT1,SEM1,CDC37,ISCU,ELF3,UBB,RPS28,CCDC85B,SELENOW,CLP1,RPL36A,SPINT2,PTOV1,NXF1,COX4I1,ACOT7,NDUFA4,SAP18,SOD1,COX6C,COX6A1,COX7A2,TCP1,PSMA6,GRN,ERCC5,GPX4,TXN,PPP1R11,SNRPF,VIPR2,RHOA,COX7B,GNAT2,EEF1D,PRDX2 FOUNDER_NAMES