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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="TIEN_INTESTINE_PROBIOTICS_2HR_DN" SYSTEMATIC_NAME="M8916" HISTORICAL_NAMES="" PMID="16394013" AUTHORS="Tien MT,Girardin SE,Regnault B,Le Bourhis L,Dillies MA,Coppée JY,Bourdet-Sicard R,Sansonetti PJ,Pédron T" GEOID="" EXACT_SOURCE="Table 1S: 2h coculture" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_HG_U133" CONTRIBUTOR="Arthur Liberzon" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes down-regulated in Caco-2 cells (intestinal epithelium) after coculture with the probiotic bacteria L. casei for 2h." DESCRIPTION_FULL="Shigella invades the human intestinal mucosa, thus causing bacillary dysentery, an acute recto-colitis responsible for lethal complications, mostly in infants and toddlers. Conversely, commensal bacteria live in a mutualistic relationship with the intestinal mucosa that is characterized by homeostatic control of innate responses, thereby contributing to tolerance to the flora. Cross-talk established between commensals and the intestinal epithelium mediate this active process, the mechanisms of which remain largely uncharacterized. Probiotics such as Lactobacillus casei belong to a subclass of these commensals that modulate mucosal innate responses and possibly display anti-inflammatory properties. We analyzed whether L. casei could attenuate the pro-inflammatory signaling induced by Shigella flexneri after invasion of the epithelial lining. Cultured epithelial cells were infected with L. casei, followed by a challenge with S. flexneri. Using macroarray DNA chips, we observed that L. casei down-regulated the transcription of a number of genes encoding pro-inflammatory effectors such as cytokines and chemokines and adherence molecules induced by invasive S. flexneri. This resulted in an anti-inflammatory effect that appeared mediated by the inhibition of the NF-kappaB pathway, particularly through stabilization of I-kappaBalpha. In a time-course experiment using GeneChip hybridization analysis, the expression of many genes involved in ubiquitination and proteasome processes were modulated during L. casei treatment. Thus, L. casei has developed a sophisticated means to maintain intestinal homeostasis through a process that involves manipulation of the ubiquitin/proteasome pathway upstream of I-kappaBalpha." TAGS="" MEMBERS="200810_s_at,200999_s_at,201244_s_at,201278_at,201280_s_at,201494_at,201569_s_at,201594_s_at,202066_at,202133_at,202176_at,202227_s_at,202371_at,202406_s_at,202552_s_at,202557_at,202623_at,202806_at,203067_at,203097_s_at,203414_at,203560_at,203607_at,203608_at,203651_at,203853_s_at,203893_at,203973_s_at,204405_x_at,204748_at,205063_at,205980_s_at,206418_at,206571_s_at,206613_s_at,208792_s_at,209030_s_at,209048_s_at,209210_s_at,209238_at,209572_s_at,210054_at,210656_at,211063_s_at,211137_s_at,211200_s_at,211458_s_at,211615_s_at,211764_s_at,212077_at,212265_at,212473_s_at,212511_at,212780_at,212824_at,212877_at,212887_at,212917_x_at,212964_at,212981_s_at,213074_at,213822_s_at,214247_s_at,214281_s_at,214306_at,214639_s_at,214749_s_at,214769_at,215695_s_at,217776_at,217819_at,217853_at,217986_s_at,218050_at,218085_at,218095_s_at,218614_at,218618_s_at,218889_at,219017_at,219099_at,219117_s_at,219250_s_at,219990_at,220239_at,221538_s_at,221763_at,32069_at,34764_at,45633_at,52285_f_at,55872_at" MEMBERS_SYMBOLIZED="AK6,ALDH5A1,ARHGAP8,ARMCX6,ATP2C1,BAZ1A,BRD8,CADM1,CALD1,CEBPD,CEP76,CHMP5,CIRBP,CKAP4,CLCN4,CLU,CRIM1,DAB2,DBN1,DIMT1,DKK3,E2F8,EAPP,EED,EFCAB2,ERCC3,ETNK1,FERMT2,FKBP11,FLRT3,FNDC3B,FUBP3,GAB2,GABARAPL1,GEMIN2,GGH,GINS3,GOLGA7,HAUS3,HIC2,HOXA1,HSPA13,INPP5F,JMJD1C,KLC1,KLHL7,LARS2,LRPPRC,MAP4K4,MICAL2,MMD,N4BP1,NCK1,NOC3L,NOX1,OPA1,PDHX,PHIP,PICALM,PLXNA1,PPFIA1,PPP4R1,PRCP,PTGS2,QKI,RAF1,RAPGEF2,RCHY1,RDH11,RECQL,RESF1,SAMM50,SEC23A,SOS1,STX3,TAF1A,TCAF1,TCEAL4,TIAL1,TIGAR,TMEM165,TNS3,UBE2D1,UBE3B,UFM1,WWTR1,ZFYVE16,ZMYND8,ZNF512B" MEMBERS_EZID="10128,102157402,10484,1052,10902,10970,10979,11177,1153,1183,1191,1601,1627,2071,221037,22876,23119,23395,23531,23613,23705,23710,23767,23779,25813,25898,25937,27032,27035,27122,27292,3198,3831,4690,4976,51109,51125,51232,51303,51510,51569,5361,54470,55023,55196,5547,55500,55837,55858,55975,57103,5743,57473,5894,5965,64318,64759,64778,64785,6654,6782,6809,7073,7321,7915,79441,79733,79921,79959,800,8050,8301,84288,8487,8500,8726,8836,8939,89910,9015,9444,9448,9645,9683,9693,9747,9765,9846,9989" MEMBERS_MAPPING="200810_s_at,CIRBP,1153|200999_s_at,CKAP4,10970|201244_s_at,RAF1,5894|201278_at,DAB2,1601|201280_s_at,DAB2,1601|201494_at,PRCP,5547|201569_s_at,SAMM50,25813|201594_s_at,PPP4R1,9989|202066_at,PPFIA1,8500|202133_at,WWTR1,25937|202176_at,ERCC3,2071|202227_s_at,BRD8,10902|202371_at,TCEAL4,79921|202406_s_at,TIAL1,7073|202552_s_at,CRIM1,51232|202557_at,HSPA13,6782|202623_at,EAPP,55837|202806_at,DBN1,1627|203067_at,PDHX,8050|203097_s_at,RAPGEF2,9693|203414_at,MMD,23531|203560_at,GGH,8836|203607_at,INPP5F,22876|203608_at,ALDH5A1,7915|203651_at,ZFYVE16,9765|203853_s_at,GAB2,9846|203893_at,AK6,102157402|203973_s_at,CEBPD,1052|204405_x_at,DIMT1,27292|204748_at,PTGS2,5743|205063_at,GEMIN2,8487|205980_s_at,ARHGAP8,23779|206418_at,NOX1,27035|206571_s_at,MAP4K4,9448|206613_s_at,TAF1A,9015|208792_s_at,CLU,1191|209030_s_at,CADM1,23705|209048_s_at,ZMYND8,23613|209210_s_at,FERMT2,10979|209238_at,STX3,6809|209572_s_at,EED,8726|210054_at,HAUS3,79441|210656_at,EED,8726|211063_s_at,NCK1,4690|211137_s_at,ATP2C1,27032|211200_s_at,EFCAB2,84288|211458_s_at,GABARAPL1,23710|211615_s_at,LRPPRC,10128|211764_s_at,UBE2D1,7321|212077_at,CALD1,800|212265_at,QKI,9444|212473_s_at,MICAL2,9645|212511_at,PICALM,8301|212780_at,SOS1,6654|212824_at,FUBP3,8939|212877_at,KLC1,3831|212887_at,SEC23A,10484|212917_x_at,RECQL,5965|212964_at,HIC2,23119|212981_s_at,TCAF1,9747|213074_at,PHIP,55023|213822_s_at,UBE3B,89910|214247_s_at,DKK3,27122|214281_s_at,RCHY1,25898|214306_at,OPA1,4976|214639_s_at,HOXA1,3198|214749_s_at,ARMCX6,54470|214769_at,CLCN4,1183|215695_s_at,null,null|217776_at,RDH11,51109|217819_at,GOLGA7,51125|217853_at,TNS3,64759|217986_s_at,BAZ1A,11177|218050_at,UFM1,51569|218085_at,CHMP5,51510|218095_s_at,TMEM165,55858|218614_at,RESF1,55196|218618_s_at,FNDC3B,64778|218889_at,NOC3L,64318|219017_at,ETNK1,55500|219099_at,TIGAR,57103|219117_s_at,FKBP11,51303|219250_s_at,FLRT3,23767|219990_at,E2F8,79733|220239_at,KLHL7,55975|221538_s_at,PLXNA1,5361|221763_at,JMJD1C,221037|32069_at,N4BP1,9683|34764_at,LARS2,23395|45633_at,GINS3,64785|52285_f_at,CEP76,79959|55872_at,ZNF512B,57473" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Homo sapiens" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
