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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="TIEN_INTESTINE_PROBIOTICS_2HR_UP" SYSTEMATIC_NAME="M15196" HISTORICAL_NAMES="" PMID="16394013" AUTHORS="Tien MT,Girardin SE,Regnault B,Le Bourhis L,Dillies MA,Coppée JY,Bourdet-Sicard R,Sansonetti PJ,Pédron T" GEOID="" EXACT_SOURCE="Table 1S: 2h coculture" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_HG_U133" CONTRIBUTOR="Arthur Liberzon" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes up-regulated in Caco-2 cells (intestinal epithelium) after coculture with the probiotic bacteria L. casei for 2h." DESCRIPTION_FULL="Shigella invades the human intestinal mucosa, thus causing bacillary dysentery, an acute recto-colitis responsible for lethal complications, mostly in infants and toddlers. Conversely, commensal bacteria live in a mutualistic relationship with the intestinal mucosa that is characterized by homeostatic control of innate responses, thereby contributing to tolerance to the flora. Cross-talk established between commensals and the intestinal epithelium mediate this active process, the mechanisms of which remain largely uncharacterized. Probiotics such as Lactobacillus casei belong to a subclass of these commensals that modulate mucosal innate responses and possibly display anti-inflammatory properties. We analyzed whether L. casei could attenuate the pro-inflammatory signaling induced by Shigella flexneri after invasion of the epithelial lining. Cultured epithelial cells were infected with L. casei, followed by a challenge with S. flexneri. Using macroarray DNA chips, we observed that L. casei down-regulated the transcription of a number of genes encoding pro-inflammatory effectors such as cytokines and chemokines and adherence molecules induced by invasive S. flexneri. This resulted in an anti-inflammatory effect that appeared mediated by the inhibition of the NF-kappaB pathway, particularly through stabilization of I-kappaBalpha. In a time-course experiment using GeneChip hybridization analysis, the expression of many genes involved in ubiquitination and proteasome processes were modulated during L. casei treatment. Thus, L. casei has developed a sophisticated means to maintain intestinal homeostasis through a process that involves manipulation of the ubiquitin/proteasome pathway upstream of I-kappaBalpha." TAGS="" MEMBERS="200031_s_at,200767_s_at,200888_s_at,200922_at,201051_at,201123_s_at,201861_s_at,205749_at,207821_s_at,208596_s_at,208615_s_at,208752_x_at,209134_s_at,209212_s_at,209468_at,210338_s_at,210735_s_at,211296_x_at,212311_at,212391_x_at,213614_x_at,213753_x_at,214167_s_at,216231_s_at,216520_s_at,216680_s_at,217356_s_at,220937_s_at,221305_s_at,49679_s_at,AFFX-BioC-5_at,AFFX-BioDn-3_at,AFFX-CreX-3_at,AFFX-CreX-5_at,AFFX-hum_alu_at,AFFX-HUMGAPDH/M33197_5_at,AFFX-r2-Ec-bioB-3_at,AFFX-r2-Ec-bioC-3_at,AFFX-r2-Ec-bioC-5_at,AFFX-r2-Ec-bioD-3_at,AFFX-r2-Ec-bioD-5_at,AFFX-r2-P1-cre-3_at,AFFX-r2-P1-cre-5_at" MEMBERS_SYMBOLIZED="ANP32A,B2M,CA12,CYP1A1,EEF1A1,EIF5A,EPHB4,FAM120A,GAPDH,HSPA8,KDELR1,KLF5,LRP5,LRRFIP1,MMP24OS,NAP1L1,PGK1,PTK2,PTP4A2,RPL23,RPLP0,RPS11,RPS3A,RPS6,SEL1L3,ST6GALNAC4,TPT1,UBC,UGT1A10,UGT1A8" MEMBERS_EZID="101410538,10945,1543,1915,1984,2050,23196,23231,2597,27090,3312,4041,4673,5230,54575,54576,567,5747,6175,6189,6194,6205,688,7178,7316,771,8073,8125,9208,9349" MEMBERS_MAPPING="200031_s_at,RPS11,6205|200767_s_at,FAM120A,23196|200888_s_at,RPL23,9349|200922_at,KDELR1,10945|201051_at,ANP32A,8125|201123_s_at,EIF5A,1984|201861_s_at,LRRFIP1,9208|205749_at,CYP1A1,1543|207821_s_at,PTK2,5747|208596_s_at,UGT1A10,54575|208615_s_at,PTP4A2,8073|208752_x_at,NAP1L1,4673|209134_s_at,RPS6,6194|209212_s_at,KLF5,688|209468_at,LRP5,4041|210338_s_at,HSPA8,3312|210735_s_at,CA12,771|211296_x_at,UBC,7316|212311_at,SEL1L3,23231|212391_x_at,RPS3A,6189|213614_x_at,EEF1A1,1915|213753_x_at,EIF5A,1984|214167_s_at,RPLP0,6175|216231_s_at,B2M,567|216520_s_at,TPT1,7178|216680_s_at,EPHB4,2050|217356_s_at,PGK1,5230|220937_s_at,ST6GALNAC4,27090|221305_s_at,UGT1A8,54576|49679_s_at,MMP24OS,101410538|AFFX-BioC-5_at,null,null|AFFX-BioDn-3_at,null,null|AFFX-CreX-3_at,null,null|AFFX-CreX-5_at,null,null|AFFX-HUMGAPDH/M33197_5_at,GAPDH,2597|AFFX-hum_alu_at,null,null|AFFX-r2-Ec-bioB-3_at,null,null|AFFX-r2-Ec-bioC-3_at,null,null|AFFX-r2-Ec-bioC-5_at,null,null|AFFX-r2-Ec-bioD-3_at,null,null|AFFX-r2-Ec-bioD-5_at,null,null|AFFX-r2-P1-cre-3_at,null,null|AFFX-r2-P1-cre-5_at,null,null" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Homo sapiens" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
