STANDARD_NAME	VIETOR_IFRD1_TARGETS
SYSTEMATIC_NAME	M1534
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/VIETOR_IFRD1_TARGETS
NAMESPACE	MOUSE_SEQ_ACCESSION
DESCRIPTION_BRIEF	Genes down-regulated in c-JunER cells (mammary gland epithelum) by overexpression of IFRD1 [GeneID=3475] off an adenovirus vector.
DESCRIPTION_FULL	The mammalian SIN3 complex consists of histone deacetylases (HDAC1, HDAC2), several known proteins (SAP30, N-CoR) and as yet unidentified proteins. Here we show that the mouse tetradecanoyl phorbol acetate induced sequence 7 (TIS7) protein is a novel transcriptional co-repressor that can associate with the SIN3 complex. We have identified tis7 as a gene that is up-regulated upon loss of polarity in a mouse mammary gland epithelial cell line expressing an estrogen-inducible c-JunER fusion protein. In unpolarized cells, TIS7 protein levels increase and TIS7 translocates into the nucleus. Overexpression of tis7 causes loss of polarity and represses a set of genes, as revealed by cDNA microarray analysis. We have shown that TIS7 protein interacts with several proteins of the SIN3 complex (mSin3B, HDAC1, N-CoR and SAP30) by yeast two-hybrid screening and co-immunoprecipitations. TIS7 co-immunoprecipitated HDAC complex is enzymatically active and represses a GAL4-dependent reporter transcription. The transcriptional repression of endogenous genes by tis7 overexpression is HDAC dependent. Thus, we propose TIS7 as a transcriptional co-repressor affecting the expression of specific genes in a HDAC activity-dependent manner during cell fate decisions, e.g. scattering.
PMID	12198164
GEOID	
AUTHORS	Vietor I,Vadivelu SK,Wick N,Hoffman R,Cotten M,Seiser C,Fialka I,Wunderlich W,Haase A,Korinkova G,Brosch G,Huber LA
CONTRIBUTOR	John Newman
CONTRIBUTOR_ORG	University of Washington
EXACT_SOURCE	Table 1: Diff. expression =< -1.5
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	AA014127,AA016730,AA033398,AA041834,AA067890,AA087137,AA105830,AA108928,AA174807,AA178015,AA190292,AA238637,AA245993,AA250500,AA434902,AA462396,AA499926,W08348,W09957,W11571,W13528,W14289,W36541,W41563,W74865,W81912
GENE_SYMBOLS	KRT7,MSX2,RPS15A,TK1,MED21,UQCR10,WFDC2,SPP1,RPL22L1,,ANP32E,FUNDC2,CTH,PTTG1,FEM1A,SEM1,PPIA,EBP,RETREG1,RPL17,MYH4,CTSZ,,ATP1A2,TOMM7,CRABP2
FOUNDER_NAMES