STANDARD_NAME WANG_SMARCE1_TARGETS_UP SYSTEMATIC_NAME M1804 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/WANG_SMARCE1_TARGETS_UP NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes up-regulated in BT549 cells (breast cancer) by expression of SMARCE1 [GeneID=6605] off a retroviral vector. DESCRIPTION_FULL Mutation of BRG1, hBRM, and their associated factors, INI1 and BAF57, in primary human tumors has suggested that inactivation of human SWI/SNF (hSWI/SNF) complexes may be involved in neoplastic transformation. BT549 is an invasive human breast carcinoma cell line that lacks expression of BAF57, a key hSWI/SNF subunit that mediates interaction with transcriptional activators and corepressors. In this study we investigated the role of BAF57 in suppressing tumorigenesis by establishing BT549 stable cell lines that expresses full-length BAF57 protein. BT549 clones expressing BAF57 demonstrated marked phenotypic changes, slow growth kinetics, and restoration of contact inhibition. Altered growth was found to be due in part to cell cycle arrest and induction of apoptosis. Furthermore, microarray analysis revealed that BAF57-mediated cell death was associated with up-regulation of proapoptotic genes including the tumor suppressor familial cylindromatosis (CYLD), which was found to be a direct target of BAF57 as determined by chromatin immunoprecipitation analysis. Increased expression of CYLD in BT549 cells induced apoptosis, while its suppression by small interfering RNA inhibited cell death in BAF57 expressing BT549 cells. These findings demonstrate the importance of BAF57 in cell growth regulation and provide a novel link between hSWI/SNF chromatin remodelers and apoptosis. PMID 16135788 GEOID AUTHORS Wang L,Baiocchi RA,Pal S,Mosialos G,Caligiuri M,Sif S CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 1S, 3S FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 200629_at,200646_s_at,200696_s_at,200878_at,200879_s_at,200974_at,201008_s_at,201050_at,201058_s_at,201060_x_at,201061_s_at,201062_at,201069_at,201117_s_at,201125_s_at,201430_s_at,201431_s_at,201438_at,201506_at,201508_at,201596_x_at,201615_x_at,201617_x_at,201668_x_at,201744_s_at,201852_x_at,201860_s_at,201888_s_at,201893_x_at,201925_s_at,201926_s_at,202007_at,202254_at,202255_s_at,202291_s_at,202310_s_at,202350_s_at,202363_at,202440_s_at,202478_at,202479_s_at,202599_s_at,202719_s_at,202720_at,202724_s_at,202746_at,202948_at,202949_s_at,203085_s_at,203167_at,203232_s_at,203325_s_at,203372_s_at,203474_at,203476_at,203477_at,203485_at,203504_s_at,203505_at,203700_s_at,203851_at,203854_at,203963_at,203989_x_at,204040_at,204058_at,204059_s_at,204072_s_at,204114_at,204201_s_at,204235_s_at,204237_at,204451_at,204452_s_at,204508_s_at,204619_s_at,204620_s_at,204627_s_at,204628_s_at,204646_at,204686_at,204749_at,204779_s_at,204955_at,205027_s_at,205105_at,205158_at,205206_at,205258_at,205268_s_at,205366_s_at,205372_at,205498_at,205539_at,205542_at,205547_s_at,205601_s_at,205679_x_at,205943_at,205991_s_at,206007_at,206067_s_at,206157_at,206209_s_at,206421_s_at,206472_s_at,206785_s_at,206825_at,207014_at,207781_s_at,207808_s_at,208490_x_at,208650_s_at,208651_x_at,208747_s_at,209030_s_at,209031_at,209098_s_at,209099_x_at,209230_s_at,209395_at,209396_s_at,209409_at,209427_at,209582_s_at,209583_s_at,209602_s_at,209604_s_at,209682_at,209719_x_at,209720_s_at,209732_at,209771_x_at,209822_s_at,209955_s_at,209990_s_at,209991_x_at,210139_s_at,210198_s_at,210413_x_at,210495_x_at,210735_s_at,210809_s_at,211161_s_at,211571_s_at,211679_x_at,211719_x_at,211813_x_at,211896_s_at,211906_s_at,211958_at,211959_at,212062_at,212154_at,212158_at,212235_at,212314_at,212344_at,212353_at,212354_at,212400_at,212464_s_at,212488_at,212489_at,212687_at,212770_at,212812_at,212822_at,213069_at,213222_at,213238_at,213397_x_at,213764_s_at,213800_at,214020_x_at,214040_s_at,214164_x_at,214390_s_at,214452_at,214587_at,214701_s_at,214725_at,215076_s_at,215112_x_at,215388_s_at,215646_s_at,215843_s_at,215867_x_at,215913_s_at,216039_at,216268_s_at,216379_x_at,216442_x_at,216973_s_at,216974_at,217077_s_at,217161_x_at,217430_x_at,217867_x_at,217892_s_at,217901_at,217963_s_at,217975_at,218113_at,218541_s_at,218559_s_at,218574_s_at,218675_at,218885_s_at,218901_at,218918_at,219054_at,219179_at,219383_at,219563_at,219729_at,219874_at,220092_s_at,220213_at,220787_at,221011_s_at,221590_s_at,221731_x_at,221796_at,221872_at,221933_at,222108_at,222392_x_at,222453_at,222455_s_at,222457_s_at,222662_at,222670_s_at,222760_at,222833_at,222853_at,223091_x_at,223143_s_at,223278_at,223354_x_at,223492_s_at,223523_at,223524_s_at,223854_at,224002_s_at,224173_s_at,224215_s_at,224436_s_at,224560_at,224595_at,224596_at,224694_at,224724_at,224741_x_at,224766_at,224793_s_at,224811_at,224819_at,224823_at,224872_at,224893_at,224937_at,224950_at,224964_s_at,224989_at,224990_at,225056_at,225144_at,225202_at,225258_at,225265_at,225274_at,225275_at,225285_at,225345_s_at,225571_at,225575_at,225602_at,225604_s_at,225664_at,225681_at,225801_at,225817_at,225897_at,225990_at,226084_at,226103_at,226145_s_at,226158_at,226189_at,226223_at,226225_at,226231_at,226237_at,226267_at,226297_at,226302_at,226415_at,226416_at,226517_at,226538_at,226545_at,226552_at,226573_at,226641_at,226676_at,226677_at,226683_at,226695_at,226756_at,226757_at,226785_at,226865_at,226899_at,226930_at,227314_at,227361_at,227461_at,227620_at,227697_at,227752_at,227812_at,227889_at,228109_at,228121_at,228176_at,228291_s_at,228328_at,228340_at,228485_s_at,228486_at,228635_at,228640_at,228653_at,228737_at,228780_at,228915_at,228988_at,229173_at,229256_at,229339_at,229450_at,229461_x_at,229513_at,229521_at,229553_at,229802_at,230030_at,230338_x_at,230383_x_at,230661_at,231202_at,231240_at,231579_s_at,231726_at,231766_s_at,231842_at,231852_at,231879_at,231964_at,232060_at,233329_s_at,233388_at,233587_s_at,233847_x_at,234432_at,234623_x_at,235000_at,235056_at,235103_at,235209_at,235210_s_at,235417_at,235467_s_at,235489_at,235766_x_at,235821_at,235852_at,235874_at,235944_at,236255_at,236277_at,236285_at,236297_at,237250_at,238516_at,238623_at,238692_at,238699_s_at,238739_at,238943_at,238999_at,239468_at,240432_x_at,240873_x_at,241359_at,241902_at,241925_x_at,242107_x_at,242258_at,242481_at,242808_at,242940_x_at,243115_at,243152_at,243813_at,244215_at,266_s_at,40093_at,60084_at GENE_SYMBOLS WARS1,NUCB1,GSN,EPAS1,EPAS1,ACTA2,TXNIP,PLD3,MYL9,STOM,STOM,,MMP2,CPE,ITGB5,DPYSL3,DPYSL3,COL6A3,TGFBI,IGFBP4,KRT18,CALD1,CALD1,MARCKS,LUM,COL3A1,PLAT,IL13RA1,DCN,CD55,CD55,NID1,SIPA1L1,SIPA1L1,MGP,COL1A1,MATN2,SPOCK1,DENND2B,TRIB2,TRIB2,NRIP1,TES,TES,FOXO1,ITM2A,IL1R1,FHL2,TGFB1,TIMP2,ATXN1,COL5A1,SOCS2,IQGAP2,TPBG,COL15A1,RTN1,ABCA1,,DIO2,IGFBP6,CFI,CA12,F2R,RNF144A,ME1,ME1,FRY,NID2,PTPN13,GULP1,GULP1,FZD1,FZD1,CA12,VCAN,VCAN,ITGB3,ITGB3,DPYD,IRS1,NAP1L3,HOXB7,SRPX,MAP3K8,MAN2A1,ANG,ANOS1,INHBB,ADD2,HOXB6,PLAG1,GHR,AVIL,STEAP1,TAGLN,HOXB5,ACAN,TDO2,PRRX1,PRG4,WT1,PTX3,CA4,SERPINB7,TLE3,KLRC1,OXTR,GABRA2,ZNF711,PROS1,H2BC7,CD24P2,CD24,C1S,CADM1,CADM1,JAG1,JAG1,NUPR1,CHI3L1,CHI3L1,GRB10,SMTN,CD200,CD200,GATA3,GATA3,CBLB,SERPINB3,SERPINB3,CLEC2B,CD24,VLDLR,FAP,GABBR2,GABBR2,PMP22,PLP1,SERPINB4,FN1,CA12,POSTN,COL3A1,VCAN,GABBR2,FN1,DCN,DCN,SERPINB4,IGFBP5,IGFBP5,ATP9A,SDC2,SDC2,PLXND1,SEL1L3,SULF1,SULF1,SULF1,EEIG1,FN1,COL5A1,COL5A1,LIMS1,TLE3,SERINC5,HEG1,HEG1,PLCB1,ATP10D,RNASE4,MFAP5,CFH,,GSN,CA12,BCAT1,BCAT1,COL8A1,FN1,SBSPON,COL3A1,MCF2L2,CFH,VCAN,TLL2,CA12,GULP1,GABRA2,JAG1,CD24,FN1,HOXB7,,GABBR2,ACAN,COL1A1,BACE2,LIMA1,DSG2,BEX3,TCEAL9,CEMIP2,TCIM,MAFB,LMCD1,SLC22A17,GALNT12,PLSCR4,MAN1C1,NPR3,DACT1,PRR5L,SYNE3,PRRX2,SLC12A8,ANTXR1,TSHZ2,,LBH,ALDH6A1,VCAN,NTRK2,RARRES1,NLGN4X,AMIGO2,PERP,CYBRD1,PARVA,LIMA1,PPP1R3B,MAFB,ZNF703,LPCAT2,FLRT3,MFF,AKIRIN2,GJB2,MFF,LRRFIP1,TMEM108,TMEM108,PCDHB10,FKBP7,MRPL30,DLL1,NIPSNAP3A,TIMP2,SLC44A1,SLC44A1,ANTXR1,SULF2,GAS5,RPL37,TGFBR1,LPP,TCEAL8,MYLK,DIP2B,ATL3,PTGFRN,PTGFRN,GNG2,SMIM14,SMIM14,SIPA1L2,BMPR2,RHOBTB3,FBLIM1,RBMS1,PCYOX1,EDIL3,BCAT1,FBXO32,LIFR,LIFR,GLIPR2,GLIPR2,COL12A1,CTHRC1,FBXO32,CGNL1,MARCKS,BOC,MAP1B,NEXN,FRAS1,KLHL24,ITGB8,PAWR,MCC,PAWR,COL8A1,JDP2,HIPK3,ATP8B1,VAT1L,ERI1,BCAT1,MAN2A1,CD109,IER5L,DIRAS1,ANKRD44,ZNF521,ZNF521,SNX18,PRRX1,CCDC71L,IFIT2,ATP11C,PLXDC2,UNC5B,FNDC1,ITGA2,HS3ST3B1,STON2,SLC44A1,SOCS3,SPTLC3,TNFRSF19,LPCAT2,RASGRF2,TGFB2,S1PR3,KIZ,KLHL28,TLE3,SLC44A1,SLC44A1,PCDH10,PCDH7,SAMD5,TOX2,POU3F3,DACH1,ZNF711,LNPK,PGM2L1,MYOCD,IFIT3,NEGR1,STRBP,CCDC71L,PGM2L1,CCN4,HS6ST2,GSX2,SLFN5,SBSPON,ALDH1L2,,TIMP2,PCDHB14,COL12A1,JCAD,ERI1,COL12A1,BICD1,ROR1,KRCC1,,SIPA1L2,,,,LPP,ETV6,MAN2A1,SBSPON,SBSPON,SPOCD1,KCNC4,RHOJ,RAB27A,CCN4,STON2,PRSS35,HMCN1,PLEKHG4B,,KLHDC7B,PLXDC2,GABRA2,BMPR2,MAP3K4-AS1,ABTB3,CASK,IPMK,FIBCD1,CTDSP2,MKX,KLF7,DAB2,TLCD2,MKX,SLC16A7,TBX18,,PCDHB2,RPL31P46,DLX6,,,LINC00968,BTD,CD24,BCAM,CYLD FOUNDER_NAMES