STANDARD_NAME WHITESIDE_CISPLATIN_RESISTANCE_DN SYSTEMATIC_NAME M8834 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/WHITESIDE_CISPLATIN_RESISTANCE_DN NAMESPACE HUMAN_SEQ_ACCESSION DESCRIPTION_BRIEF Genes down-regulated in NCI-H2170 cells (lung cancer) upon induction of resistance to cisplatin [PubChem=2767]. DESCRIPTION_FULL In recent years, most cDNA microarray studies of chemotherapeutic drug resistance have not considered the temporal pattern of gene expression. The objective of this study was to examine systematically changes in gene expression of NCI-H226 and NCI-H2170 lung cancer cells treated weekly with IC10 doses of cisplatin. NCI-H226 lung cancer cells were treated weekly with an IC10 dose of cisplatin. Candidate genes with a fold change of 2.0 or more were identified from this study. A second experiment was conducted by exposing NCI-H2170 cells to cisplatin doses that were increased in week 4 and decreased in week 5. Overall, 44 genes were differentially expressed in both the NCI-H226 and NCI-H2170 cell lines. In the NCI-H2170 cell line, 24 genes had a twofold gene expression change from weeks 3 to 4. Real-time PCR found a significant correlation of the gene expression changes for seven genes of interest. This small time-ordered series identified novel genes associated with cisplatin resistance. This kind of analysis should be viewed as a first step towards building gene-regulatory networks. PMID 14737109 GEOID AUTHORS Whiteside MA,Chen DT,Desmond RA,Abdulkadir SA,Johanning GL CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 1: week 4 < 1 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS L04483,M13267,M60854,U14970,U14971,U32907,X00351,X02492,X03124,X56681,X56932,X82200,Y00503 GENE_SYMBOLS RPS21,SOD1,RPS16,RPS5,RPS9,LRRC17,ACTB,IFI6,TIMP1,JUND,RPL13A,TRIM22,KRT19 FOUNDER_NAMES