STANDARD_NAME ZHANG_PROLIFERATING_VS_QUIESCENT SYSTEMATIC_NAME M16992 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/ZHANG_PROLIFERATING_VS_QUIESCENT NAMESPACE HUMAN_SEQ_ACCESSION DESCRIPTION_BRIEF Genes up-regulated in HDMEC cells (microvascular endothelium): proliferating vs quiescent cells. DESCRIPTION_FULL Endothelial cells are known to be a rich source of transcriptional gene expression. Recent technological advances now permit the detailed profiling of mRNA expression using arrays of known cDNAs on blots. We have used such arrays to examine expression of mRNA by primary isolates of human foreskin microvascular endothelial cells in the proliferative and quiescent state. Cells were stimulated by a combination of known growth factors for these cells including epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and 'endothelial cell growth supplement (ECGS)' either alone or in combination. Analysis showed the expression of many mRNAs but of the 588 examined, only one, namely monocyte chemotactic protein-1 (MCP-1), showed a decrease on treatment with EGF. A combination of image grabbing followed by subtractive densitometry enabled identification of the mRNAs upregulated in proliferating endothelium. In consideration of the possibility of selective vascular targeting, of particular interest was the increase in expression of the mRNA for the cell surface proteins vascular endothelial (VE-) and neural (N-) cadherin and alpha5, alphav, beta1 and beta3 integrins. The alpha5 integrin offers a previously unrecognized opportunity for vascular targeting. PMID 14517420 GEOID AUTHORS Zhang HT,Gorn M,Smith K,Graham AP,Lau KK,Bicknell R CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 2: Differential expression=arrow up FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS D13639,D15057,D21090,J02703,J04111,K00065,K03515,L06139,L16785,L19067,L19185,L22474,L25080,L29511,L32976,L34587,M14631,M14648,M21304,M25753,M26708,M28882,M33374,M34064,M35410,M60974,M76125,M81757,M82234,M92381,M92934,S40706,U08839,U13737,U28014,U32944,U47414,X06256,X07270,X07979,X08020,X15218,X15480,X53586,X53799,X54079,X59798,X60957,X69391,X70326,Y00787,Z12020 GENE_SYMBOLS CCND2,DAD1,RAD23B,ITGB3,JUN,,GPI,TEK,NME2,RELA,PRDX2,BAX,RHOA,GRB2,MAP3K11,ELOC,GNAS,ITGAV,GPX1,CCNB1,PTMA,MCAM,NDUFB7,CDH2,IGFBP2,GADD45A,AXL,RPS19,,TMSB10,CCN2,DDIT3,PLAUR,CASP3,CASP4,DYNLL1,CCNG2,ITGA5,HSP90AA1,,GSTM1,SKI,GSTP1,ITGA6,CXCL2,HSPB1,CCND1,TIE1,RPL6,MARCKSL1,CXCL8,MDM2 FOUNDER_NAMES