STANDARD_NAME ZWANG_EGF_PERSISTENTLY_DN SYSTEMATIC_NAME M2621 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/ZWANG_EGF_PERSISTENTLY_DN NAMESPACE AFFY_HuGene DESCRIPTION_BRIEF Genes persistently repressed by EGF [GeneID =1950] in 184A1 cells (mammary epithelium). DESCRIPTION_FULL Normal cells require continuous exposure to growth factors in order to cross a restriction point and commit to cell-cycle progression. This can be replaced by two short, appropriately spaced pulses of growth factors, where the first pulse primes a process, which is completed by the second pulse, and enables restriction point crossing. Through integration of comprehensive proteomic and transcriptomic analyses of each pulse, we identified three processes that regulate restriction point crossing: (1) The first pulse induces essential metabolic enzymes and activates p53-dependent restraining processes. (2) The second pulse eliminates, via the PI3K/AKT pathway, the suppressive action of p53, as well as (3) sets an ERK-EGR1 threshold mechanism, which digitizes graded external signals into an all-or-none decision obligatory for S phase entry. Together, our findings uncover two gating mechanisms, which ensure that cells ignore fortuitous growth factors and undergo proliferation only in response to consistent mitogenic signals. PMID 21596316 GEOID GSE27629 AUTHORS Zwang Y,Sas-Chen A,Drier Y,Shay T,Avraham R,Lauriola M,Shema E,Lidor-Nili E,Jacob-Hirsch J,Amariglio N,Lu Y,Mills GB,Rechavi G,Oren M,Domany E,Yarden Y CONTRIBUTOR Yaara Zwang CONTRIBUTOR_ORG Broad Institute EXACT_SOURCE Table 2S: Persistently repressed FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 7898809,7899244,7901175,7901613,7909446,7915659,7929689,7937892,7939942,7942889,7945663,7945873,7949033,7949046,7949746,7949904,7950701,7952274,7952436,7955663,7956031,7967325,7968344,7968787,7971241,7973530,7974229,7975971,7983173,7987554,8008454,8009243,8009746,8012423,8015946,8020973,8034122,8034454,8034521,8041826,8042574,8045887,8048014,8052382,8059413,8062251,8063187,8063814,8069744,8075616,8079462,8082248,8087236,8088776,8099326,8099860,8100338,8104422,8111101,8116724,8118734,8121563,8121749,8138145,8138151,8144281,8156319,8160835,8169504,8180240 GENE_SYMBOLS EPHB2,PIGV,TSPAN1,ACOT11,HSD11B1,HECTD3,MARVELD1,PGAP2,OR5D14,TMEM126A,IFITM10,OR7E21P,MACROD1,TRPT1,POLD4,UNC93B1,TENM4,NECTIN1,ESAM,TNS2,ORMDL2,HCAR1,ALOX5AP,OR7E21P,OR7E21P,PCK2,KLHDC2,SAMD15,CCNDBP1,DNAJC17,ABCC3,MILR1,SLC16A5,CTC1,ASB16-AS1,FHOD3,SPC24,DHPS,HOOK2,TTC7A,OR7E21P,OR7E21P,RPE,,DOCK10,EPB41L1,EYA2,LSM14B,RWDD2B,SYN3,NBEAL2,OR7E15P,DALRD3,FOXP1,SLC2A9,RFC1,ERVMER34-1,MTRR,ANKH,FARS2,ITPR3,MARCKS,GJA1,OR7E21P,OR7E21P,ARHGEF10,OR7E15P,RPP25L,SLC6A14,EOLA1-DT FOUNDER_NAMES