Systematic name M12948
Brief description Genes down-regulated in MCF7 cells (breast cancer) after treatment with the oxydants: hydrogen peroxyde, menadione, and t-butyl hydroperoxyde [PubChem=784;4055;6410].
Full description or abstract Global gene expression patterns in breast cancer cells after treatment with oxidants (hydrogen peroxide, menadione, and t-butyl hydroperoxide) were investigated in three replicate experiments. RNA collected after treatment (at 1, 3, 7, and 24 h) rather than after a single time point, enabled an analysis of gene expression patterns. Using a 17,000 microarray, template-based clustering and multidimensional scaling analysis of the gene expression over the entire time course identified 421 genes as being either up- or down-regulated by the three oxidants. In contrast, only 127 genes were identified for any single time point and a 2-fold change criteria. Surprisingly, the patterns of gene induction were highly similar among the three oxidants; however, differences were observed, particularly with respect to p53, IL-6, and heat-shock related genes. Replicate experiments increased the statistical confidence of the study, whereas changes in gene expression patterns over a time course demonstrated significant additional information versus a single time point. Analyzing the three oxidants simultaneously by template cluster analysis identified genes that heretofore have not been associated with oxidative stress.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 12414654   Authors: Chuang YY,Chen Y,Gadisetti,Chandramouli VR,Cook JA,Coffin D,Tsai MH,DeGraff W,Yan H,Zhao S,Russo A,Liu ET,Mitchell JB
Exact source Table 3: ratio < 0
Related gene sets (show 1 additional gene sets from the source publication)

(show 2461 gene sets from the same authors)
External links  
Filtered by similarity ?
Organism Homo sapiens
Contributed by John Newman (University of Washington)
Source platform HUMAN_GENE_SYMBOL
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 11 genes
Gene families ? Categorize these 11 genes by gene family
Show members (show 11 members mapped to 11 genes)
Version history 3.0: Renamed from OXSTRESS_BREASTCA_DN

See MSigDB license terms here. Please note that certain gene sets have special access terms.