Human Gene Set: FEST_POST_ACTIVATED_B_CELL_CD23_UP


Standard name FEST_POST_ACTIVATED_B_CELL_CD23_UP
Systematic name M50216
Brief description Post activated B cell that maintain the IL4-STAT6 CD23 signaling and that are diverted from plasma cell differentiation
Full description or abstract In late B-cell differentiation, a cell can become a post-activated B cell or produce antibody-secreting cells. We showed that B cells that had committed to becoming PBs downregulate expression of the CD23 cell surface marker. This finding enabled us to characterize the split in the cell fate by comparing P2/CD23+ and P2/CD23- populations using RNA-sequencing. Three paired experiments were performed with D4lo, P2/CD23+, P2/CD23- and P1 cells (cultured and sorted) from independent blood donors. Libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina), and samples were sequenced on an Illumina NextSeq 500 High Output v2 system (Illumina) using 75-bp single-end reads. Differential expression of filtered genes (HTSfilter v1.7.1, 11803 genes) was analyzed using DESeq2 (version 1.12.4) in R (version 3.3.1). Differentially expressed genes were defined as those with an adjusted p-value <0.05 and a fold change >2 after Wald's test and Benjamini Hochberg correction for multiple testing. When D4lo, P2/CD23+, and P2/CD23- populations were compared for IL-4 response signatures by GSEA, P2/ CD23 positive showed the greatest enrichment. The actual signature FEST_CD23_UP correspond o the genes up-regulated in P2/CD23+ population. Hence, this population sustained IL-4 response thus maintaining the cells in an activated state and dampened their capacity to differentiate.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 33150420   Authors: Pignarre A,Chatonnet F,Caron G,Haas M,Desmots F,Fest T
Exact source Fig2A P2/CD23 UP sub-population & supplemental Figure 4A & B
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Source species Homo sapiens
Contributed by FEST Thierry (INSERM)
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identifier namespace		 HUMAN_GENE_SYMBOL
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Version history 2026.1.Hs: First Introduced.


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