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Human Gene Set: HARALAMBIEVA_PBMC_M_M_R_II_AGE_13_16YO
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| Standard name | HARALAMBIEVA_PBMC_M_M_R_II_AGE_13_16YO_STIMULATED_VS_UNSTIMULATED_3_TO_7YR_POST_TOP_DEG_UP |
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| Systematic name | M41076 |
| Brief description | Genes up-regulated in peripheral blood mononuclear cell stimulated vs unstimulated in children (13-16) after exposure to M-M-R II , time point 3 to 7Y. Comment: top differentially expressed genes, full set of 1080 avail in Suppl Materials |
| Full description or abstract | Immune responses to current rubella vaccines demonstrate significant inter-individual variability. We performed mRNA-Seq profiling on PBMCs from high and low antibody responders to rubella vaccination to delineate transcriptional differences upon viral stimulation. Generalized linear models were used to assess the per gene fold change (FC) for stimulated versus unstimulated samples or the interaction between outcome and stimulation. Model results were evaluated by both FC and p-value. Pathway analysis and self-contained gene set tests were performed for assessment of gene group effects. Of 17,566 detected genes, we identified 1,080 highly significant differentially expressed genes upon viral stimulation (p < 1.00E(-15), FDR < 1.00E(-14)), including various immune function and inflammation-related genes, genes involved in cell signaling, cell regulation and transcription, and genes with unknown function. Analysis by immune outcome and stimulation status identified 27 genes (p <= 0.0006 and FDR <= 0.30) that responded differently to viral stimulation in high vs. low antibody responders, including major histocompatibility complex (MHC) class I genes (HLA-A, HLA-B and B2M with p = 0.0001, p = 0.0005 and p = 0.0002, respectively), and two genes related to innate immunity and inflammation (EMR3 and MEFV with p = 1.46E(-08) and p = 0.0004, respectively). Pathway and gene set analysis also revealed transcriptional differences in antigen presentation and innate/inflammatory gene sets and pathways between high and low responders. Using mRNA-Seq genome-wide transcriptional profiling, we identified antigen presentation and innate/inflammatory genes that may assist in explaining rubella vaccine-induced immune response variations. Such information may provide new scientific insights into vaccine-induced immunity useful in rational vaccine development and immune response monitoring. |
| Collection | C7: Immunologic Signature VAX: HIPC Vaccine Response |
| Source publication | Pubmed 23658707 Authors: Haralambieva IH,Oberg AL,Ovsyannikova IG,Kennedy RB,Grill DE,Middha S,Bot BM,Wang VW,Smith DI,Jacobson RM,Poland GA |
| Exact source | Table 2 |
| Related gene sets |
(show 36 gene sets from the same authors)
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| External links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641062/table/pone-0062149-t002/ |
| Filtered by similarity ? | |
| Source species | Homo sapiens |
| Contributed by | HIPC SIGNATURES (NIAID/HIPC SIGNATURES) |
| Source platform or identifier namespace |
HUMAN_GENE_SYMBOL |
| Dataset references | |
| Download gene set | format: grp | gmt | xml | json | TSV metadata |
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| Compendia expression profiles ? |
NG-CHM interactive heatmaps (Please note that clustering takes a few seconds) Legacy heatmaps (PNG) |
| Advanced query | Further investigate these 42 genes |
| Gene families ? | Categorize these 42 genes by gene family |
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(show 42 source identifiers mapped to 42 genes)
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| Version history | 7.3: First Introduced. |
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