Standard name |
MISHRA_CARCINOMA_ASSOCIATED_FIBROBLAST_DN |
Systematic name |
M4577 |
Brief description |
Top genes down-regulated in mesenchyme stem cells (MSC) grown in a tumor conditioned medium, which leads to carcinoma-associated fibroblast phenotype. |
Full description or abstract |
Carcinoma-associated fibroblasts (CAF) have recently been implicated in important aspects of epithelial solid tumor biology, such as neoplastic progression, tumor growth, angiogenesis, and metastasis. However, neither the source of CAFs nor the differences between CAFs and fibroblasts from nonneoplastic tissue have been well defined. In this study, we show that human bone marrow-derived mesenchymal stem cells (hMSCs) exposed to tumor-conditioned medium (TCM) over a prolonged period of time assume a CAF-like myofibroblastic phenotype. More importantly, these cells exhibit functional properties of CAFs, including sustained expression of stromal-derived factor-1 (SDF-1) and the ability to promote tumor cell growth both in vitro and in an in vivo coimplantation model, and expression of myofibroblast markers, including alpha-smooth muscle actin and fibroblast surface protein. hMSCs induced to differentiate to a myofibroblast-like phenotype using 5-azacytidine do not promote tumor cell growth as efficiently as hMSCs cultured in TCM nor do they show increased SDF-1 expression. Furthermore, gene expression profiling revealed similarities between TCM-exposed hMSCs and CAFs. Taken together, these data suggest that hMSCs are a source of CAFs and can be used in the modeling of tumor-stroma interactions. To our knowledge, this is the first report showing that hMSCs become activated and resemble carcinoma-associated myofibroblasts on prolonged exposure to conditioned medium from MDAMB231 human breast cancer cells. |
Collection |
C2: Curated CGP: Chemical and Genetic Perturbations |
Source publication |
Pubmed 18519693 Authors: Mishra PJ,Mishra PJ,Humeniuk R,Medina DJ,Alexe G,Mesirov JP,Ganesan S,Glod JW,Banerjee D |
Exact source |
p. 4335: Top 25 (of 47) down-regulated genes |
Related gene sets |
(show 1 additional gene sets from the source publication)
(show 113 gene sets from the same authors)
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External links |
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Filtered by similarity ?
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Source species |
Homo sapiens |
Contributed by |
Leona Saunders (MSigDB Team) |
Source platform or identifier namespace |
HUMAN_GENE_SYMBOL |
Dataset references |
(show 1 datasets)
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Download gene set |
format: grp | gmt | xml | json | TSV metadata |
Compute overlaps ? |
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Compendia expression profiles ? |
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
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Advanced query |
Further investigate
these 24 genes
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Gene families ? |
Categorize these
24 genes by gene family
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Show members |
(show 24 source identifiers mapped to 24 genes)
Source Id |
NCBI (Entrez) Gene Id |
Gene Symbol |
Gene Description |
BDNF |
627 |
BDNF |
brain derived neurotrophic factor [Sour... |
C1S |
716 |
C1S |
complement C1s [Source:HGNC Symbol;Acc:... |
CAT |
847 |
CAT |
catalase [Source:HGNC Symbol;Acc:HGNC:1... |
CD24 |
100133941 |
CD24 |
CD24 molecule [Source:HGNC Symbol;Acc:H... |
CD55 |
1604 |
CD55 |
CD55 molecule (Cromer blood group) [Sou... |
FTL |
2512 |
FTL |
ferritin light chain [Source:HGNC Symbo... |
HGF |
3082 |
HGF |
hepatocyte growth factor [Source:HGNC S... |
HSPA1A |
3303 |
HSPA1A |
heat shock protein family A (Hsp70) mem... |
ID2 |
3398 |
ID2 |
inhibitor of DNA binding 2 [Source:HGNC... |
JUP |
3728 |
JUP |
junction plakoglobin [Source:HGNC Symbo... |
KRT14 |
3861 |
KRT14 |
keratin 14 [Source:HGNC Symbol;Acc:HGNC... |
KRT18 |
3875 |
KRT18 |
keratin 18 [Source:HGNC Symbol;Acc:HGNC... |
KRT19 |
3880 |
KRT19 |
keratin 19 [Source:HGNC Symbol;Acc:HGNC... |
KRT7 |
3855 |
KRT7 |
keratin 7 [Source:HGNC Symbol;Acc:HGNC:... |
KRT8 |
3856 |
KRT8 |
keratin 8 [Source:HGNC Symbol;Acc:HGNC:... |
MAN2B1 |
4125 |
MAN2B1 |
mannosidase alpha class 2B member 1 [So... |
MET |
4233 |
MET |
MET proto-oncogene, receptor tyrosine k... |
MFAP4 |
4239 |
MFAP4 |
microfibril associated protein 4 [Sourc... |
MGST1 |
4257 |
MGST1 |
microsomal glutathione S-transferase 1 ... |
PPGB |
5476 |
CTSA |
cathepsin A [Source:HGNC Symbol;Acc:HGN... |
RARRES2 |
5919 |
RARRES2 |
retinoic acid receptor responder 2 [Sou... |
SFRP2 |
6423 |
SFRP2 |
secreted frizzled related protein 2 [So... |
SPINT2 |
10653 |
SPINT2 |
serine peptidase inhibitor, Kunitz type... |
TCEAL1 |
9338 |
TCEAL1 |
transcription elongation factor A like ... |
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Version history |
3.0: First introduced
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