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Human Gene Set: RICHERT_PBMC_HIV_LIPO_5_AGE_37_48YO
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| Standard name | RICHERT_PBMC_HIV_LIPO_5_AGE_37_48YO_STIMULATED_VS_UNSTIMULATED_14W_TOP_FUNCTIONAL_NETWORK_UP |
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| Systematic name | M41035 |
| Brief description | Genes up-regulated in peripheral blood mononuclear cell stimulated vs unstimulated in adults (37-48) after exposure to HIV-LIPO-5 , time point 14W. Comment: top functional network of up-regulated genes |
| Full description or abstract | OBJECTIVE: To dissect the biological mechanisms involved in the cellular responses to a candidate vaccine containing 5 HIV peptides coupled to a palmytoil tail (HIV-LIPO-5) in healthy volunteers, by using extensive immunogenicity assessments with different stimulation durations. DESIGN: Immunogenicity substudy of a randomized phase II prophylactic HIV vaccine trial (ANRS VAC 18). METHODS: HIV-LIPO-5 or placebo was administered at W0, W4, W12 and W24. Peripheral blood mononuclear cells from a subset of participants at W0 and W14 were stimulated with HIV-LIPO-5, Gag peptides contained in the vaccine and control peptides. ELISpot, lymphoproliferation, intracellular cytokine staining (ICS), cytokine multiplex and transcriptomic analyses were performed. Different time points and stimulation conditions were compared, controlling for test multiplicity. RESULTS: Cultured ELISpot and lymphoproliferation responses were detected at W14. Ex-vivo ICS showed mainly interleukin (IL)-2-producing cells. Secretion of interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha, IL-5 and IL-13 increased significantly after culture and Gag stimulation at W14 compared to W0. Metallothionein genes were consistently overexpressed after HIV-LIPO-5 stimulation at W0 and W14. At W14, significant probes increased substantially, including IFN-gamma, CXCL9, IL2RA, TNFAIP6, CCL3L1 and IL-6. Canonical pathway analyses indicated a role of interferon signalling genes in response to HIV-LIPO-5. CONCLUSION: HIV-LIPO-5 vaccination elicited Th1 and Th2 memory precursor responses and a consistent modulation in gene expression. The response profile before vaccination suggests an adjuvant effect of the lipid tail of HIV-LIPO-5. Our combined immunogenicity analyses allowed to identify a specific signature profile of HIV-LIPO-5 and indicate that HIV-LIPO-5 could be further developed as a prime in heterologous prime-boost strategies. |
| Collection | C7: Immunologic Signature VAX: HIPC Vaccine Response |
| Source publication | Pubmed 23759749 Authors: Richert L,Hue S,Hocini H,Raimbault M,Lacabaratz C,Surenaud M,Wiedemann A,Tisserand P,Durier C,Salmon D,Lelièvre JD,Chêne G,Thiébaut R,Lévy Y,ANRS Vaccine Network/Vaccine Research Institute |
| Exact source | Suppl Fig 6 |
| Related gene sets |
(show 3 additional gene sets from the source publication)
(show 1 gene sets from the same authors) |
| External links | http://links.lww.com/QAD/A314 |
| Filtered by similarity ? | |
| Source species | Homo sapiens |
| Contributed by | HIPC SIGNATURES (NIAID/HIPC SIGNATURES) |
| Source platform or identifier namespace |
HUMAN_GENE_SYMBOL |
| Dataset references | |
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| Advanced query | Further investigate these 26 genes |
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(show 27 source identifiers mapped to 26 genes)
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| Version history | 7.3: First Introduced. |
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