Systematic name M2891
Brief description Genes down-regulated in T lymphocytes overexpressing TERT [GeneID=7015] off a retrovirus vector.
Full description or abstract Little is known about the long-term consequences of overexpression of the human telomerase reverse transcriptase (hTERT) gene in T lymphocytes. To address this issue, we transduced polyclonal as well as clonally derived populations of naive and memory CD44 T cells from 2 healthy donors (aged 24 and 34 years) with retroviral vectors encoding green fluorescence protein (GFP) and hTERT (GFP-hTERT) or GFP alone. After transduction, cells were sorted on the basis of GFP expression and cultured in vitro until senescence. T cells transduced with hTERT exhibited high stable telomerase activity throughout the culture period. Relative to GFP controls, minor changes in overall gene expression were observed yet the proliferative lifespan of the hTERT-transduced populations was significantly increased and the rate of telomere loss was lower. Nevertheless, hTERT-transduced cells showed progressive telomere loss and had shorter telomeres at senescence than controls (2.3 +/- 0.3 kilobase [kb] versus 3.4 +/- 0.1 kb). Furthermore, a population of cells with 4N DNA consisting of binucleated cells with connected nuclei emerged in the hTERT-transduced cells prior to senescence. We conclude that overexpression of hTERT in CD4+ T cells provides a proliferative advantage independent of the average telomere length but does not prevent eventual genetic instability and replicative senescence.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 15741219   Authors: Röth A,Baerlocher GM,Schertzer M,Chavez E,Dührsen U,Lansdorp PM
Exact source Table 2
Related gene sets (show 1 additional gene sets from the source publication)

(show 4 gene sets from the same authors)
External links  
Filtered by similarity ?
Organism Homo sapiens
Contributed by Kevin Vogelsang (MSigDB Team)
Source platform AFFY_HG_U133
Dataset references (show 1 datasets)
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 9 genes
Gene families ? Categorize these 9 genes by gene family
Show members (show 11 members mapped to 9 genes)
Version history  

See MSigDB license terms here. Please note that certain gene sets have special access terms.