Human Gene Set: STK33_DN

Standard name STK33_DN
Systematic name M2853
Brief description Genes down-regulated in NOMO-1 and SKM-1 cells (AML) after knockdown of STK33 [GeneID=65975] by RNAi.
Full description or abstract NOMO-1 and SKM-1 acute myeloid leukemia (AML) cells were stably transduced with different pLKO.1puro lentiviral shRNA vectors targeting STK33 or a nontargeting control shRNA. VSV-G-pseudotyped lentiviral particles were produced by cotransfection of 293T cells with pLKO.1 constructs and the compatible packaging plasmids pMD.G and pCMVR8.91. Virus was harvested 48 and 72 hours after transfection, cells were incubated with lentiviral supernatants for 30 hours, and infected cells were selected with 2ug/ml puromycin. RNA was isolated after 2 days of puromycin selection and isolation of viable cells by density gradient centrifugation, and gene expression was profiled using GeneChip Human Genome U133 Plus 2.0 microarrays (Affymetrix).
Collection C6: Oncogenic Signature
Source publication Pubmed 19490892   Authors: Scholl C,Fröhling S,Dunn IF,Schinzel AC,Barbie DA,Kim SY,Silver SJ,Tamayo P,Wadlow RC,Ramaswamy S,Döhner K,Bullinger L,Sandy P,Boehm JS,Root DE,Jacks T,Hahn WC,Gilliland DG
Exact source control vs KD STK33 in nomo & skm cells; bottom 300 genes (diff. of means)
Related gene sets (show 5 additional gene sets from the source publication)

(show 444 gene sets from the same authors)
External links
Filtered by similarity ?
Source species Homo sapiens
Contributed by Pablo Tamayo (Broad Institute)
Source platform or
identifier namespace
Dataset references (show 1 datasets)
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 280 genes
Gene families ? Categorize these 280 genes by gene family
Show members (show 300 source identifiers mapped to 280 genes)
Version history  

We need your help: Update on GSEA/MSigDB funding support

Last November we submitted a proposal to NCI's Information Technology for Cancer Research (ITCR) program for the continued funding of GSEA and MSigDB. Unfortunately, our proposal was not funded in this round, but we were encouraged to resubmit for the next one. This funding is critical for our continuing support and enhancement of the GSEA-MSigDB resource.

For our original submission many of you sent us emails of support, an important requirement for these grants. We now ask for your help again. We would greatly appreciate a short email message from you describing how the resource has been of value to your work and any concerns you may have about its continued availability.

Please send us your message of support to on or before Monday June 5, 2023.

Thanks in advance for your help and support.
The GSEA/MSigDB Team.

See MSigDB license terms here. Please note that certain gene sets have special access terms.