Human Gene Set: STK33_SKM_UP

Standard name STK33_SKM_UP
Systematic name M2857
Brief description Genes up-regulated in SKM-1 cells (AML) after knockdown of STK33 [GeneID=65975] by RNAi.
Full description or abstract SKM-1 acute myeloid leukemia (AML) cells were stably transduced with different pLKO.1puro lentiviral shRNA vectors targeting STK33 or a nontargeting control shRNA. VSV-G-pseudotyped lentiviral particles were produced by cotransfection of 293T cells with pLKO.1 constructs and the compatible packaging plasmids pMD.G and pCMVR8.91. Virus was harvested 48 and 72 hours after transfection, cells were incubated with lentiviral supernatants for 30 hours, and infected cells were selected with 2ug/ml puromycin. RNA was isolated after 2 days of puromycin selection and isolation of viable cells by density gradient centrifugation, and gene expression was profiled using GeneChip Human Genome U133 Plus 2.0 microarrays (Affymetrix).
Collection C6: Oncogenic Signature
Source publication Pubmed 19490892   Authors: Scholl C,Fröhling S,Dunn IF,Schinzel AC,Barbie DA,Kim SY,Silver SJ,Tamayo P,Wadlow RC,Ramaswamy S,Döhner K,Bullinger L,Sandy P,Boehm JS,Root DE,Jacks T,Hahn WC,Gilliland DG
Exact source control vs KD STK33 in skm cells; top 300 genes (diff. of means)
Related gene sets (show 5 additional gene sets from the source publication)

(show 444 gene sets from the same authors)
External links
Filtered by similarity ?
Source species Homo sapiens
Contributed by Pablo Tamayo (Broad Institute)
Source platform or
identifier namespace
Dataset references (show 1 datasets)
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)

Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 279 genes
Gene families ? Categorize these 279 genes by gene family
Show members (show 300 source identifiers mapped to 279 genes)
Version history  

See MSigDB license terms here. Please note that certain gene sets have special access terms.