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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Mm" NAME="export">
    <GENESET STANDARD_NAME="CASTELLANO_HRAS_AND_NRAS_TARGETS_DN" SYSTEMATIC_NAME="MM467" HISTORICAL_NAMES="" PMID="16909116" AUTHORS="Castellano E,De Las Rivas J,Guerrero C,Santos E" GEOID="" EXACT_SOURCE="Table 3: delta(i) &lt; 0" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_MG_U74" CONTRIBUTOR="Leona Saunders" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes down-regulated in MEF cells (embryonic fibroblasts) isolated from HRAS and NRAS [GeneID=3265;4893] double knockout mice." DESCRIPTION_FULL="We characterized differential gene expression profiles of fibroblast cell lines harboring single or double-homozygous null mutations in H-ras and N-ras. Whereas the expression level of the individual H-, N- and K-ras genes appeared unaffected by the presence or absence of the other ras loci, significant differences were observed between the expression profiles of cells missing N-ras and/or H-ras. Absence of N-ras produced much stronger effects than absence of H-ras over the profile of the cellular transcriptome. N-ras(-/-) and H-ras(-/-) fibroblasts displayed rather antagonistic expression profiles and the transcriptome of H-ras(-/-) cells was significantly closer to that of wild-type fibroblasts than to that of N-ras(-/-) cells. Classifying all differentially expressed genes into functional categories suggested specific roles for H-Ras and N-Ras. It was particularly striking in N-ras(-/-) cells the upregulation of a remarkable number of immunity-related genes, as well as of several loci involved in apoptosis. Reverse-phase protein array assays demonstrated in the same N-ras(-/-) cells the overexpression and nuclear migration of tyrosine phosphorylated signal transducer and activator of transcription 1 (Stat1) which was concomitant with transcriptional activation mediated by interferon-stimulated response elements. Significantly enhanced numbers of apoptotic cells were also detected in cultures of N-ras(-/-) cells. Our data support the notion that different Ras isoforms play functionally distinct cellular roles and indicate that N-Ras is significantly involved in immune modulation/host defense and apoptotic responses." TAGS="" MEMBERS="101156_at,102048_at,102399_at,103888_at,93748_at,94362_at,95701_at,97498_at,97795_at,99034_at" MEMBERS_SYMBOLIZED="Ankrd1,Calcrl,Cxxc5,Fhl1,Irx3,Npn2,Nras,Polr2m,Rbpms" MEMBERS_EZID="107765,14199,16373,18153,18176,19663,28015,54598,67393" MEMBERS_MAPPING="101156_at,Npn2,18153|102048_at,Ankrd1,107765|102399_at,Rbpms,19663|103888_at,Rbpms,19663|93748_at,Polr2m,28015|94362_at,Nras,18176|95701_at,Cxxc5,67393|97498_at,Fhl1,14199|97795_at,Calcrl,54598|99034_at,Irx3,16373" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="M2" ORGANISM="Mus musculus" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
