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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Mm" NAME="export">
    <GENESET STANDARD_NAME="RIZ_ERYTHROID_DIFFERENTIATION_6HR" SYSTEMATIC_NAME="MM512" HISTORICAL_NAMES="" PMID="17213805" AUTHORS="Riz I,Akimov SS,Eaker SS,Baxter KK,Lee HJ,Mariño-Ramírez L,Landsman D,Hawley TS,Hawley RG" GEOID="" EXACT_SOURCE="Table 6S" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_MG_U74" CONTRIBUTOR="Leona Saunders" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Selected genes down-regulated in the TLX1 [GeneID=3195] Tet On iEBHX15-4 cells (pro-erythroblasts) at 6 h time point." DESCRIPTION_FULL="Aberrant expression of the human homeobox-containing proto-oncogene TLX1/HOX11 inhibits hematopoietic differentiation programs in a number of murine model systems. Here, we report the establishment of a murine erythroid progenitor cell line, iEBHX1S-4, developmentally arrested by regulatable TLX1 expression. Extinction of TLX1 expression released the iEBHX1S-4 differentiation block, allowing erythropoietin-dependent acquisition of erythroid markers and hemoglobin synthesis. Coordinated activation of erythroid transcriptional networks integrated by the acetyltransferase co-activator CREB-binding protein (CBP) was suggested by bioinformatic analysis of the upstream regulatory regions of several conditionally induced iEBHX1S-4 gene sets. In accord with this notion, CBP-associated acetylation of GATA-1, an essential regulator of erythroid differentiation, increased concomitantly with TLX1 downregulation. Coimmunoprecipitation experiments and glutathione-S-transferase pull-down assays revealed that TLX1 directly binds to CBP, and confocal laser microscopy demonstrated that the two proteins partially colocalize at intranuclear sites in iEBHX1S-4 cells. Notably, the distribution of CBP in conditionally blocked iEBHX1S-4 cells partially overlapped with chromatin marked by a repressive histone methylation pattern, and downregulation of TLX1 coincided with exit of CBP from these heterochromatic regions. Thus, we propose that TLX1-mediated differentiation arrest may be achieved in part through a mechanism that involves redirection of CBP and/or its sequestration in repressive chromatin domains." TAGS="" MEMBERS="101162_at,101396_at,101904_at,102048_at,102087_at,102579_f_at,102668_at,102955_at,103283_at,104240_at,104590_at,160604_at,160731_at,161418_r_at,161584_r_at,162314_at,92248_at,92255_at,92344_at,92535_at,92614_at,92717_at,92883_at,92891_f_at,92894_s_at,92896_s_at,92902_at,92999_at,93230_at,93371_at,93418_g_at,93613_at,94100_s_at,94636_at,94710_g_at,95391_at,95584_at,95968_at,97357_at,98780_at,99058_at,99427_at,99432_at,99965_at" MEMBERS_SYMBOLIZED="Ankrd1,Bnc1,Clip2,Cux1,Dppa2,Ebf1,Elf5,Foxc1,Gcm2,Hltf,Hmga2,Hnf1b,Hoxa13,Hoxa3,Hoxa6,Hoxb3,Hoxb4,Hoxc9,Hoxd12,Id3,Mef2b,Mef2c,Mybl1,Myf5,Neurod1,Neurog1,Nfia,Nfil3,Nr4a2,Ppara,Rab1a,Rab25,Sim2,Six4,Smad3,Trpc4,Vax1,Vdr" MEMBERS_EZID="107765,107889,12173,13047,13591,13711,15364,15398,15400,15403,15410,15412,15427,15432,15903,17127,17259,17260,17300,17864,17877,18012,18014,18027,18030,18227,19013,19324,20465,20474,20585,21410,22066,22326,22337,269713,53868,73703" MEMBERS_MAPPING="101162_at,Myf5,17877|101396_at,Hnf1b,21410|101904_at,null,null|102048_at,Ankrd1,107765|102087_at,Hoxa3,15400|102579_f_at,Hoxa6,15403|102668_at,Ppara,19013|102955_at,Nfil3,18030|103283_at,Elf5,13711|104240_at,Cux1,13047|104590_at,Mef2c,17260|160604_at,Foxc1,17300|160731_at,Rab25,53868|161418_r_at,null,null|161584_r_at,null,null|162314_at,null,null|92248_at,Nr4a2,18227|92255_at,Hoxb4,15412|92344_at,Hltf,20585|92535_at,Ebf1,13591|92614_at,Id3,15903|92717_at,Neurod1,18012|92883_at,Rab1a,19324|92891_f_at,Hoxc9,15427|92894_s_at,Nfia,18027|92896_s_at,Sim2,20465|92902_at,Mybl1,17864|92999_at,Six4,20474|93230_at,Neurog1,18014|93371_at,Bnc1,12173|93418_g_at,Mef2b,17259|93613_at,Smad3,17127|94100_s_at,Trpc4,22066|94636_at,Hoxa13,15398|94710_g_at,Gcm2,107889|95391_at,Vax1,22326|95584_at,Dppa2,73703|95968_at,null,null|97357_at,Mef2c,17260|98780_at,Hoxb3,15410|99058_at,Hmga2,15364|99427_at,Hoxd12,15432|99432_at,Clip2,269713|99965_at,Vdr,22337" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="M2" ORGANISM="Mus musculus" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
