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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Mm" NAME="export">
    <GENESET STANDARD_NAME="RIZ_ERYTHROID_DIFFERENTIATION_HEMGN" SYSTEMATIC_NAME="MM510" HISTORICAL_NAMES="" PMID="17213805" AUTHORS="Riz I,Akimov SS,Eaker SS,Baxter KK,Lee HJ,Mariño-Ramírez L,Landsman D,Hawley TS,Hawley RG" GEOID="" EXACT_SOURCE="Table 4S" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_MG_U74" CONTRIBUTOR="Leona Saunders" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Selected gradually up-regulated genes whose expression profile follows that of HEMGN [GeneID=55363] in the TLX1 [GeneID=3195] Tet On iEBHX15-4 cells (pro-erythroblasts)." DESCRIPTION_FULL="Aberrant expression of the human homeobox-containing proto-oncogene TLX1/HOX11 inhibits hematopoietic differentiation programs in a number of murine model systems. Here, we report the establishment of a murine erythroid progenitor cell line, iEBHX1S-4, developmentally arrested by regulatable TLX1 expression. Extinction of TLX1 expression released the iEBHX1S-4 differentiation block, allowing erythropoietin-dependent acquisition of erythroid markers and hemoglobin synthesis. Coordinated activation of erythroid transcriptional networks integrated by the acetyltransferase co-activator CREB-binding protein (CBP) was suggested by bioinformatic analysis of the upstream regulatory regions of several conditionally induced iEBHX1S-4 gene sets. In accord with this notion, CBP-associated acetylation of GATA-1, an essential regulator of erythroid differentiation, increased concomitantly with TLX1 downregulation. Coimmunoprecipitation experiments and glutathione-S-transferase pull-down assays revealed that TLX1 directly binds to CBP, and confocal laser microscopy demonstrated that the two proteins partially colocalize at intranuclear sites in iEBHX1S-4 cells. Notably, the distribution of CBP in conditionally blocked iEBHX1S-4 cells partially overlapped with chromatin marked by a repressive histone methylation pattern, and downregulation of TLX1 coincided with exit of CBP from these heterochromatic regions. Thus, we propose that TLX1-mediated differentiation arrest may be achieved in part through a mechanism that involves redirection of CBP and/or its sequestration in repressive chromatin domains." TAGS="" MEMBERS="101305_at,101310_at,102643_at,102715_at,102882_at,103204_r_at,103229_at,103990_at,104292_at,104408_s_at,160109_at,161340_r_at,161521_at,161858_f_at,161965_r_at,162016_f_at,92249_g_at,92342_at,92697_at,92705_at,92781_at,92926_at,92933_at,92935_at,93616_g_at,97123_at,97704_at,97777_at,97937_at,98073_at,99527_at" MEMBERS_SYMBOLIZED="Cux1,E2f8,Ell2,Eya2,Fosb,Foxa1,Hoxa2,Klf5,Mpl,Ncoa1,Nfe2l3,Nkx2-5,Nr0b2,Nr2f1,Nr4a2,Pbx3,Pou2f3,Pou3f3,Runx1t1,Sox1,Sox18,Sox4,Tal2,Tbx2,Tmpo,Zfp46" MEMBERS_EZID="108961,12224,12395,13047,13865,14049,14282,15375,15399,17480,17977,18025,18091,18227,18516,18988,18993,192657,20664,20672,20677,21350,21385,21917,22704,23957" MEMBERS_MAPPING="101305_at,Pou3f3,18993|101310_at,Tal2,21350|102643_at,Hoxa2,15399|102715_at,Nr2f1,13865|102882_at,Zfp46,22704|103204_r_at,E2f8,108961|103229_at,Ncoa1,17977|103990_at,Fosb,14282|104292_at,Eya2,14049|104408_s_at,Sox18,20672|160109_at,Sox4,20677|161340_r_at,null,null|161521_at,null,null|161858_f_at,null,null|161965_r_at,null,null|162016_f_at,null,null|92249_g_at,Nr4a2,18227|92342_at,Sox1,20664|92697_at,Foxa1,15375|92705_at,Tbx2,21385|92781_at,Tmpo,21917|92926_at,Mpl,17480|92933_at,Pou2f3,18988|92935_at,Runx1t1,12395|93616_g_at,Pbx3,18516|97123_at,Nr0b2,23957|97704_at,Ell2,192657|97777_at,Nkx2-5,18091|97937_at,Klf5,12224|98073_at,Cux1,13047|99527_at,Nfe2l3,18025" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="M2" ORGANISM="Mus musculus" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
