Mouse Gene Set: HESS_TARGETS_OF_HOXA9_AND_MEIS1_DN

For the Human gene set with the same name, see HESS_TARGETS_OF_HOXA9_AND_MEIS1_DN

Standard name HESS_TARGETS_OF_HOXA9_AND_MEIS1_DN
Systematic name MM1009
Brief description Genes down-regulated in hematopoietic precursor cells conditionally expressing HOXA9 and MEIS1 [GeneID=3205;4211].
Full description or abstract Abdominal-type HoxA genes in combination with Meis1 are well-documented on-cogenes in various leukemias but it is unclear how they exert their transforming function. Here we used a system of conditional transformation by an inducible mixed lineage leukemia-eleven-nineteen leukemia (MLL-ENL) oncoprotein to overexpress Hoxa9 and Meis1 in primary hematopoietic cells. Arrays identified c-Myb and a c-Myb target (Gstm1) among the genes with the strongest response to Hoxa9/Meis1. c-Myb overexpression was verified by Northern blot and quantitative reverse transcription-polymerase chain reaction (RT-PCR). Also MLL-ENL activated c-Myb through up-regulation of Hoxa9 and Meis1. Consequently, short-term suppression of c-Myb by small inhibitory RNA (siRNA) efficiently inhibited transformation by MLL-ENL but did not impair transformation by transcription factor E2A-hepatic leukemia factor (E2A-HLF). The anti c-Myb siRNA effect was abrogated by coexpression of a c-Myb derivative with a mutated siRNA target site. The introduction of a dominant-negative c-Myb mutant had a similar but weaker effect on MLL-ENL-mediated transformation. Hematopoietic precursors from mice homozygous for a hypo-morphic c-Myb allele were more severely affected and could be transformed neither by MLL-ENL nor by E2A-HLF. Ectopic expression of c-Myb induced a differentiation block but c-Myb alone was not transforming in a replating assay similar to Hoxa9/Meis1. These results suggest that c-Myb is essential but not sufficient for Hoxa9/Meis1 mediated transformation.
Collection M2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 16507773   Authors: Hess JL,Bittner CB,Zeisig DT,Bach C,Fuchs U,Borkhardt A,Frampton J,Slany RK
Exact source Table 2S
Related gene sets (show 1 additional gene sets from the source publication)

(show 2 gene sets from any of these authors)
External links
Filtered by similarity ?
Source species Mus musculus
Contributed by Kevin Vogelsang (MSigDB Team)
Source platform or
identifier namespace		 AFFY_Mouse430
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
Mouse Transcriptomic BodyMap compendium

Legacy heatmaps (PNG)
Mouse Transcriptomic BodyMap compendium
Advanced query Further investigate these 86 genes
Show members (show 91 source identifiers mapped to 86 genes)
Version history 2022.1.Mm: First Introduced.

See MSigDB license terms here. Please note that certain gene sets have special access terms.