Mouse Gene Set: WANG_P53WT_LUAD_DN


Standard name WANG_P53WT_LUAD_DN
Systematic name MM19752
Brief description Genes downregulated with restoration of wild-type p53 in a lung adenocarcinoma cell line derived from a Kras driven lung adenocarcinoma mouse model
Full description or abstract This gene signature was generated from RNA sequencing data from LUAD cell lines derived from KrasLA2/+; p53LSL-p53wt/LSL-p53wt mice, in which spontaneous recombination of a latent KrasG12D allele drives LUAD. In this model, the lung tumors that form and the cell lines derived from them are effectively p53 null because of the presence of the upstream stop cassette silencing p53. Restoration of wild-type p53 in the cell lines can be achieved through the transduction of CMV-Cre adenoviruses (Ad-Cre). Infecting with Ad-Empty provides a p53 null control. Three cell lines with restored wild-type p53 (via Ad-Cre transduction) and three p53-null (via Ad-Empty transduction) were used for RNA sequencing to identify genes downregulated upon wild-type p53 restoration.
Collection M2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 41115251   Authors: Wang M,Bieging-Rolett KT,Kaiser AM,Brady CA,Lockhart JH,Ferreira S,Nguyen KT,Rajeevan A,Evans SA,Zhao T,Raj N,Elkrief A,Tischfield SE,Ladanyi M,Ozawa MG,Bui NQ,Chen CT,Flores ER,Attardi LD
Exact source Supplemental Table S7: Genes repressed 48hr after p53 restoration in LUAD cells, padj<0.01.
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External links https://aacrjournals.org/cancerdiscovery/article/doi/10.1158/2159-8290.CD-25-0525/76673...
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Source species Mus musculus
Contributed by Laura D. Attardi (Stanford University)
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identifier namespace		 MOUSE_GENE_SYMBOL
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Version history 2026.1.Mm: First Introduced.


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