Gene Set: GSE5589_IL6_KO_VS_IL10_KO_LPS_AND_IL10_STIM_MACROPHAGE_180MIN_DN

Standard name GSE5589_IL6_KO_VS_IL10_KO_LPS_AND_IL10_STIM_MACROPHAGE_180MIN_DN
Systematic name M6671
Brief description Genes down-regulated in bone marrow-derived macrophagesat 180 min of stimulation by IL10 [GeneID=3486] and LPS: IL6 [GeneID=3469] knockout versus IL10 [GeneID=3486] knockout.
Full description or abstract IL-10 or IL-6 stimulation of control 129xC57BL/6 murine bone marrow derived macrophages in the presence of LPS. We used microarrays to detail the global programme of gene expression changes in response to IL-6 or IL-10 stimulation in the presence of lipopolysaccharide. BMDMs were isolated from control, IL-6-/-, and IL-10-/- mice on a 129XBL/6 mixed background mice and differentiated in the presence of CSF-1 for 6-7 days. Cells were scraped and plated in 6 well plates at 2x10e6/well. Cells were washed with complete DMEM and rested for 1-2 hr before stimulation with combinations of IL-10 (10 ng/ml), IL-6 (2 ng/ml) or LPS (100 ng/ml) for 45 min or 180 mins. Complete biological replicates were performed.
Collection C7: immunologic signature gene sets
      IMMUNESIGDB: ImmuneSigDB gene sets
Source publication Pubmed 17114459   Authors: Kasmi El KC,Holst J,Coffre M,Mielke L,Pauw de A,Lhocine N,Smith AM,Rutschman R,Kaushal D,Shen Y,Suda T,Donnelly RP,Myers MG,Alexander W,Vignali DA,Watowich SS,Ernst M,Hilton DJ,Murray PJ
Exact source GSE5589_3018_200_DN
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(show 92 gene sets from the same authors)
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Organism Mus musculus
Contributed by Jernej Godec (Dana-Farber Cancer Institute)
Source platform HUMAN_GENE_SYMBOL
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Version history 7.3: Moved to ImmuneSigDB sub-collection.

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