Gene Set: PARK_HSC_VS_MULTIPOTENT_PROGENITORS_DN

Standard name PARK_HSC_VS_MULTIPOTENT_PROGENITORS_DN
Systematic name M1403
Brief description Genes down-regulated in long term hematopoietic stem cells (LT-HSC) compared to multipotent progenitor (MPP) cells.
Full description or abstract Hematopoietic stem cells (HSCs) have self-renewal capacity and multilineage developmental potentials. The molecular mechanisms that control the self-renewal of HSCs are still largely unknown. Here, a systematic approach using bioinformatics and array hybridization techniques to analyze gene expression profiles in HSCs is described. To enrich mRNAs predominantly expressed in uncommitted cell lineages, 54 000 cDNA clones generated from a highly enriched population of HSCs and a mixed population of stem and early multipotent progenitor (MPP) cells were arrayed on nylon membranes (macroarray or high-density array), and subtracted with cDNA probes derived from mature lineage cells including spleen, thymus, and bone marrow. Five thousand cDNA clones with very low hybridization signals were selected for sequencing and further analysis using microarrays on glass slides. Two populations of cells, HSCs and MPP cells, were compared for differential gene expression using microarray analysis. HSCs have the ability to self-renew, while MPP cells have lost the capacity for self-renewal. A large number of genes that were differentially expressed by enriched populations of HSCs and MPP cells were identified. These included transcription factors, signaling molecules, and previously unknown genes.
Collection C2: curated gene sets
      CGP: chemical and genetic perturbations
Source publication Pubmed 11781229   Authors: Park IK,He Y,Lin F,Laerum OD,Tian Q,Bumgarner R,Klug CA,Li K,Kuhr C,Doyle MJ,Xie T,Schummer M,Sun Y,Goldsmith A,Clarke MF,Weissman IL,Hood L,Li L
Exact source Table 2: low rhodamine (MPP)
Related gene sets (show 3 additional gene sets from the source publication)

(show 177 gene sets from the same authors)
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Organism Mus musculus
Contributed by Kate Stafford (MSigDB Team)
Source platform MOUSE_SEQ_ACCESSION
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Version history 3.1: First introduced

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