Human Gene Set: STK33_SKM_DN


Standard name STK33_SKM_DN
Systematic name M2856
Brief description Genes down-regulated in SKM-1 cells (AML) after knockdown of STK33 [GeneID=65975] by RNAi.
Full description or abstract SKM-1 acute myeloid leukemia (AML) cells were stably transduced with different pLKO.1puro lentiviral shRNA vectors targeting STK33 or a nontargeting control shRNA. VSV-G-pseudotyped lentiviral particles were produced by cotransfection of 293T cells with pLKO.1 constructs and the compatible packaging plasmids pMD.G and pCMVR8.91. Virus was harvested 48 and 72 hours after transfection, cells were incubated with lentiviral supernatants for 30 hours, and infected cells were selected with 2ug/ml puromycin. RNA was isolated after 2 days of puromycin selection and isolation of viable cells by density gradient centrifugation, and gene expression was profiled using GeneChip Human Genome U133 Plus 2.0 microarrays (Affymetrix).
Collection C6: Oncogenic Signature
Source publication Pubmed 19490892   Authors: Scholl C,Fröhling S,Dunn IF,Schinzel AC,Barbie DA,Kim SY,Silver SJ,Tamayo P,Wadlow RC,Ramaswamy S,Döhner K,Bullinger L,Sandy P,Boehm JS,Root DE,Jacks T,Hahn WC,Gilliland DG
Exact source control vs KD STK33 in skm cells; bottom 300 genes (diff. of means)
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Source species Homo sapiens
Contributed by Pablo Tamayo (Broad Institute)
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identifier namespace		 HUMAN_GENE_SYMBOL
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